Glioma is the most common primary brain tumor in the human brain. The present study was designed to explore the expression of PIMREG in glioma and its relevance to the clinicopathological features and prognosis of the glioma patients. The correlations of PIMREG with the infiltrating levels of immune cells and its relevance to the response to immunotherapy were also investigated. PIMREG expression in glioma was analyzed based on the GEO, TCGA, and HPA databases. Kaplan-Meier survival analysis was used to examine the predictive value of PIMREG for the prediction of prognosis in patients with glioma. The correlation between the infiltrating levels of immune cells in glioma and PIMREG was analyzed using the CIBERSORT algorithm and TIMRE database. The correlation between PIMREG and immune checkpoints and its correlation with the patients’ responses to immunotherapy were analyzed using R software and GEPIA dataset. Cell experiments were conducted to verify the action of PIMREG in glioma cell migration and invasion. We founded that PIMREG expression was up-regulated in gliomas and positively associated with WHO grade. High PIMREG expression was correlated to poor prognosis of LGG, prognosis of all WHO grade glioma, and prognosis of recurrent glioma. PIMREG was related to the filtration of several immune cells, such as M1 and M2 macrophages, monocyte and CD8+ T cells. Moreover, PIMREG was correlated with immune checkpoints in glioma and correlated with patients’ responses to immunotherapy. KEGG pathway enrichment and GO functional analysis illustrated that PIMREG was related to multiple tumor- and immune- related pathways. In conclusion, PIMREG overexpressed in gliomas was associated with poor prognosis of patients with glioma and related to immune cell infiltrates and the responses to immunotherapy.

神经胶质瘤是人脑中最常见的原发性脑肿瘤。本研究旨在探讨 PIMREG 在胶质瘤中的表达及其与胶质瘤患者临床病理特征和预后的相关性。还研究了 PIMREG 与免疫细胞浸润水平的相关性及其与免疫治疗反应的相关性。基于 GEO、TCGA 和 HPA 数据库分析胶质瘤中的 PIMREG 表达。Kaplan-Meier 生存分析用于检查 PIMREG 对胶质瘤患者预后的预测价值。采用CIBERSORT算法和TIMRE​​数据库分析胶质瘤免疫细胞浸润水平与PIMREG的相关性。使用R软件和GEPIA数据集分析PIMREG与免疫检查点之间的相关性及其与患者对免疫治疗反应的相关性。进行细胞实验以验证PIMREG在胶质瘤细胞迁移和侵袭中的作用。我们发现 PIMREG 表达在胶质瘤中上调，并且与 WHO 分级呈正相关。PIMREG 高表达与 LGG 的不良预后、所有 WHO 级胶质瘤的预后和复发性胶质瘤的预后相关。PIMREG 与几种免疫细胞的过滤有关，例如 M1 和 M2 巨噬细胞、单核细胞和 CD8+ T 细胞。此外，PIMREG 与胶质瘤中的免疫检查点相关，并与患者对免疫治疗的反应相关。KEGG通路富集和GO功能分析表明PIMREG与多种肿瘤和免疫相关通路有关。总之，在胶质瘤中过表达的 PIMREG 与胶质瘤患者的不良预后相关，并且与免疫细胞浸润和对免疫治疗的反应有关。