Binding of drugs to blood serum proteins can influence both therapeutic efficacy and toxicity. The ability to measure the concentrations of protein-bound drug molecules quickly and with limited sample preparation could therefore have considerable benefits in biomedical and pharmaceutical applications. Vibrational spectroscopies provide data quickly but are hampered by complex, overlapping protein amide I band profiles and water absorption. Here, we show that two-dimensional infrared (2D-IR) spectroscopy can achieve rapid detection and quantification of paracetamol binding to serum albumin in blood serum at physiologically-relevant levels with no additional sample processing. By measuring changes to the amide I band of serum albumin caused by structural and dynamic impacts of paracetamol binding we show that drug concentrations as low as 7 μM can be detected and that the availability of albumin for paracetamol binding is less than 20% in serum samples, allowing identification of paracetamol levels consistent with a patient overdose.

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使用 2D-IR 光谱法检测对乙酰氨基酚与血清中白蛋白的结合

药物与血清蛋白的结合会影响治疗效果和毒性。因此，在有限的样品制备过程中快速测量蛋白质结合药物分子浓度的能力可能在生物医学和制药应用中具有相当大的优势。振动光谱可快速提供数据，但会受到复杂、重叠的蛋白质酰胺 I 带谱和吸水性的阻碍。在这里，我们展示了二维红外 (2D-IR) 光谱可以实现快速检测和量化对乙酰氨基酚与血清中血清白蛋白在生理相关水平的结合，而无需额外的样品处理。