This study reports the construction of a novel SPME fiber based on chitosan and glutaraldehyde as coating material composites combined with high-performance liquid chromatography with an ultraviolet detector (HPLC-UV) for extraction and detection of phenobarbital. In this technique, the chitosan biopolymer, as a new coating of SPME fiber, was produced on the stainless-steel wire, using glutaraldehyde and phenobarbital as cross-linker and template, respectively. For comparison, a nonimprinted polymer was created using the same procedure to evaluate fiber selectivity (but without the addition of phenobarbital). The SPME-MIP fiber coating was characterized by field emission scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermal gravimetric analysis. The efficiency of fiber was then improved by adjusting the impact of numerous factors such as pH, extraction time, desorption time, desorption solvent, and stirring rate. The results showed that the proposed fiber has a linear range of 0.01–4 μg·mL⁻¹, and detection limit of 7.5 ng·mL⁻¹. The average recoveries in the four concentration levels for the spiked river and well water samples were 95.7 and 95.3%, with relative standard deviations of 3.8 and 5.9% for single fiber and between fibers, respectively.

中文翻译：

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壳聚糖作为苯巴比妥固相微萃取选择性吸附剂的分子印迹聚合物的制备

本研究报道了一种基于壳聚糖和戊二醛作为涂层材料复合材料的新型固相微萃取纤维的构建，结合高效液相色谱和紫外检测器（HPLC-UV）用于苯巴比妥的提取和检测。在该技术中，壳聚糖生物聚合物作为一种新型的固相微萃取纤维涂层，分别使用戊二醛和苯巴比妥作为交联剂和模板，在不锈钢丝上制备。为了比较，使用相同的程序创建了一种非印迹聚合物来评估纤维选择性（但不添加苯巴比妥）。通过场发射扫描电子显微镜、傅里叶变换红外光谱和热重分析对 SPME-MIP 纤维涂层进行了表征。然后通过调整许多因素的影响，如 pH 值、提取时间、解吸时间、解吸溶剂和搅拌速度，提高了纤维的效率。结果表明，所提出的光纤的线性范围为 0.01-4 μ g·mL ^-1，检出限7.5 ng·mL ^-1。加标河流和井水样品的四个浓度水平的平均回收率为 95.7% 和 95.3%，单根纤维和纤维间的相对标准偏差分别为 3.8% 和 5.9%。