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Inactivation of a wheat protein kinase gene confers broad-spectrum resistance to rust fungi
Cell ( IF 64.5 ) Pub Date : 2022-07-14 , DOI: 10.1016/j.cell.2022.06.027
Ning Wang 1 , Chunlei Tang 2 , Xin Fan 2 , Mengying He 2 , Pengfei Gan 2 , Shan Zhang 2 , Zeyu Hu 2 , Xiaodong Wang 2 , Tong Yan 2 , Weixue Shu 2 , Ligang Yu 2 , Jinren Zhao 2 , Jiani He 2 , Lili Li 2 , Jianfeng Wang 2 , Xueling Huang 3 , Lili Huang 4 , Jian-Min Zhou 5 , Zhensheng Kang 2 , Xiaojie Wang 2
Affiliation  

Wheat crops are frequently devastated by pandemic stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). Here, we identify and characterize a wheat receptor-like cytoplasmic kinase gene, TaPsIPK1, that confers susceptibility to this pathogen. PsSpg1, a secreted fungal effector vital for Pst virulence, can bind TaPsIPK1, enhance its kinase activity, and promote its nuclear localization, where it phosphorylates the transcription factor TaCBF1d for gene regulation. The phosphorylation of TaCBF1d switches its transcriptional activity on the downstream genes. CRISPR-Cas9 inactivation of TaPsIPK1 in wheat confers broad-spectrum resistance against Pst without impacting important agronomic traits in two years of field tests. The disruption of TaPsIPK1 leads to immune priming without constitutive activation of defense responses. Taken together, TaPsIPK1 is a susceptibility gene known to be targeted by rust effectors, and it has great potential for developing durable resistance against rust by genetic modifications.



中文翻译:

小麦蛋白激酶基因的失活赋予对锈病真菌的广谱抗性

小麦作物经常被条锈菌引起的大流行性条锈病所破坏。sp。小麦( Pst )。在这里,我们鉴定并表征了一种小麦受体样细胞质激酶基因TaPsIPK1,它赋予对该病原体的易感性。PsSpg1 是一种对Pst毒力至关重要的分泌型真菌效应子,它可以结合 TaPsIPK1,增强其激酶活性,并促进其核定位,在此处磷酸化转录因子 TaCBF1d 以进行基因调控。TaCBF1d 的磷酸化会改变其对下游基因的转录活性。小麦中TaPsIPK1的CRISPR-Cas9 失活赋予对Pst的广谱抗性在两年的田间试验中不影响重要的农艺性状。TaPsIPK1的破坏导致免疫启动而没有防御反应的本构激活。综上所述,TaPsIPK1是一种已知的锈病效应基因靶向的易感基因,具有通过基因改造开发持久抗锈病的巨大潜力。

更新日期:2022-07-14
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