Opiates recreational consumption has always been a concern in society, public health, and in clinical toxicology analysis. The aim of this study was to develop and fully validate an analytical method, which was simple and rapid for the determination of tramadol, codeine, morphine, 6- acetylcodeine, 6-monoacetylmorphine and fentanyl using gas chromatography coupled to tandem mass spectrometry. The procedure includes the use of microextraction by packed sorbent for sample clean-up. A mixed mode sorbent was used, allowing the minimal use of solvents. The method was validated in urine samples, with the ability to detect and quantify all analytes with satisfactory linearity (in the range of 1 – 1000 ng/mL for all analytes, except for fentanyl (10–1000 ng/mL)). Extraction efficiency varied from 17 to 107%, which did not impair sensitivity, taking into account the low LLOQs obtained (1 ng/ mL for all analytes; and 10 ng/mL for fentanyl). The developed procedure proved to be fast, selective, and accurate for use in routine analysis, with a low volume of sample (250 µL).

鸦片类消遣性消费一直是社会、公共卫生和临床毒理学分析的关注点。本研究的目的是开发和充分验证一种分析方法，该方法简单、快速，用于使用气相色谱-串联质谱法测定曲马多、可待因、吗啡、6-乙酰可待因、6-单乙酰吗啡和芬太尼。该程序包括使用填充吸附剂进行微萃取来净化样品。使用混合模式吸附剂，允许最少使用溶剂。该方法在尿液样本中得到验证，能够以令人满意的线性检测和定量所有分析物（所有分析物的范围为 1 – 1000 ng/mL，芬太尼 (10–1000 ng/mL) 除外）。提取效率从 17% 到 107% 不等，这不会影响灵敏度，考虑到获得的低 LLOQ（所有分析物为 1 ng/mL；芬太尼为 10 ng/mL）。事实证明，开发的程序快速、选择性和准确，可用于常规分析，样品量低 (250 µL)。