Since the red blood cell shape affects the oxygen transport, so a robust method to reconstruct the 3D shape of an RBC from different projections is presented. A robust one-piece polarizing holographic microscope setup is used to record inline holograms of normal and cancerous red blood cells (RBCs) with high stability. The inline holograms are corrected by flat fielding and windowed Fourier filtering methods to mitigate the zero-order and the defocused twin image due to the inline recording configuration to the least measure. The corrected inline holograms are then reconstructed by the angular spectrum method to extract the 2D wrapping phase-contrast images. The 2D wrapping phase-contrast images are then unwrapped using the graph cuts algorithm to extract the continuous 2D phase-contrast images. The continuous 2D phase-contrast images are reconstructed at different projections by the multiplicative technique to extract the 3D shape of the normal and the cancerous RBCs. Experimental results show that any deformation in the shape of the normal and the cancerous RBCs can be seen clearly at any rotational angle in 3D. This method, which is based on the degree of deformation from the best fitting, can be used as an alternative method of counting method for discrimination between normal and cancerous cells and hence diagnoses the disease easily.

中文翻译：

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使用数字全息断层扫描法重建正常和癌变红细胞的 3D 形状：角谱法和乘法技术的结合

由于红细胞形状会影响氧气运输，因此提出了一种从不同投影重建红细胞 3D 形状的稳健方法。坚固的一体式偏光全息显微镜装置用于以高稳定性记录正常和癌变红细胞 (RBC) 的在线全息图。在线全息图通过平场和窗口傅立叶滤波方法进行校正，以将在线记录配置导致的零级和散焦双像减少到最小程度。然后通过角谱方法重建校正后的在线全息图，以提取 2D 环绕相位对比图像。然后使用图形切割算法对 2D 包裹相衬图像进行展开，以提取连续的 2D 相衬图像。通过乘法技术在不同的投影处重建连续的 2D 相衬图像，以提取正常和癌性红细胞的 3D 形状。实验结果表明，正常和癌变红细胞形状的任何变形都可以在 3D 中的任何旋转角度清晰地看到。该方法基于最佳拟合的变形程度，可用作区分正常细胞和癌细胞的计数方法的替代方法，从而轻松诊断疾病。