Introduction

Given that denervation atrophy often occurs in muscle after peripheral nerve injury, the effects of injections of human adipose-derived stem cells (hADSCs) and platelet-rich plasma (PRP) into muscle after peripheral nerve injury were examined.

Methods

hADSCs were isolated from human subcutaneous fat tissue, and PRP was prepared from rat whole blood before injection into a rat sciatic nerve injury model. Muscle atrophy was evaluated by quantitating the gross musculature and muscle fiber area and walking footprint analysis.

Results

At 4 weeks post-surgery, there were significant differences in the sciatic functional index between experimental (injected with hADSCs, PRP, or combined hADSCs + PRP) and non-operated groups (p < 0.0001), but no significant differences were observed between the different treatment groups (p > 0.05). Post hoc Bonferroni tests also showed significant differences in the wet muscle weight ratios of hADSC, PRP, and combined groups compared to PBS group. The gastrocnemius muscle fiber area was larger in hADSC group and the combined group compared to PBS group at 4 weeks post-surgery.

Conclusion

The injection of hADSCs delays muscular atrophy after sciatic nerve injury in rats; thus, hADSCs are a promising alternative for regenerating atrophied muscle.

中文翻译：

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人类脂肪干细胞延缓大鼠外周神经损伤后的肌肉萎缩

介绍

鉴于周围神经损伤后肌肉经常发生去神经萎缩，研究了在周围神经损伤后将人脂肪干细胞 (hADSCs) 和富含血小板的血浆 (PRP) 注射到肌肉中的效果。

方法

从人皮下脂肪组织中分离hADSCs，从大鼠全血制备PRP，然后注射到大鼠坐骨神经损伤模型中。通过量化总肌肉组织和肌纤维面积以及步行足迹分析来评估肌肉萎缩。

结果

术后 4 周，实验组（注射 hADSCs、PRP 或联合 hADSCs + PRP）与非手术组的坐骨神经功能指数存在显着差异（p  < 0.0001），但两组之间未观察到显着差异。不同治疗组（p  > 0.05）。事后 Bonferroni 测试还显示，与 PBS 组相比，hADSC、PRP 和联合组的湿肌肉重量比存在显着差异。术后4周hADSC组及联合组腓肠肌纤维面积均大于PBS组。

结论

大鼠坐骨神经损伤后注射hADSCs延缓肌肉萎缩 因此，hADSCs 是再生萎缩肌肉的有希望的替代品。