Glycomics aims to identify the structure and function of the glycome, the complete set of oligosaccharides (glycans), produced in a given cell or organism, as well as to identify genes and other factors that govern glycosylation. This challenging endeavor requires highly robust, sensitive, and potentially automatable analytical technologies for the analysis of hundreds or thousands of glycomes in a timely manner (termed high-throughput glycomics). This review provides a historic overview as well as highlights recent developments and challenges of glycomic profiling by the most prominent high-throughput glycomic approaches, with N-glycosylation analysis as the focal point. It describes the current state-of-the-art regarding levels of characterization and most widely used technologies, selected applications of high-throughput glycomics in deciphering glycosylation process in healthy and disease states, as well as future perspectives.

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高通量糖组学方法

糖组学旨在鉴定糖组（在给定细胞或生物体中产生的完整寡糖（聚糖））的结构和功能，以及鉴定控制糖基化的基因和其他因素。这项具有挑战性的工作需要高度稳健、灵敏且具有潜在自动化能力的分析技术，以便及时分析数百或数千个糖组（称为高通量糖组学）。本综述提供了历史概述，并重点介绍了最著名的高通量糖组方法在糖组分析方面的最新发展和挑战，其中N-糖基化分析为焦点。它描述了当前最先进的表征水平和最广泛使用的技术、高通量糖组学在破译健康和疾病状态下糖基化过程中的选定应用，以及未来的前景。