Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) is an entomopathogenic fungus that has potential as a biological control agent against many insect pests. This study was conducted to optimize the conditions for large-scale production on rice of B. bassiana and evaluate its virulence against the bean flower thrips Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae). The optimal substrate quantity for conidia production was 200 g of rice per container (2.1 l), with substrate having a 52% moisture content, being supplemented with 2% cooking oil. In stage production of a batch, 150 ml of conidia suspension (at 24.33 × 107 spores/ml) was incubated for 10 days in SDA liquid medium amended with 4% glucose and 1.5% yeast extract. The SDA medium was then used to inoculate one container of the rice substrate. The optimum ratio of conidial suspension to substrate (v/v) was 20–25%. Virulence of the spore powder harvested from the rice substrate was same as that of produced on the SDA solid media against thrips. The level of control from an application of spore powder of the thrips M. usitatus was similar to that from chemical the pesticide spinetoram when applied in a cowpea (Vigna sinensis Endl) field. The optimized rice substrate system can be used for a large-scale production of B. bassiana spores, which can be used for field control of thrips.

中文翻译：

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筛选白僵菌 (Bals.) (Hypocreales: Cordycipitaceae) 菌株对抗 Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) 和大规模生产的条件

Beauveria bassiana (Bals.) (Hypocreales: Cordycipitaceae) 是一种昆虫病原真菌，具有作为生物防治许多害虫的潜力。本研究旨在优化B. bassiana 水稻大规模生产的条件，并评估其对豆花蓟马Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) 的毒力。生产分生孢子的最佳基质量是每个容器（2.1 升）200 克大米，基质含水量为 52%，辅以 2% 的食用油。在批次生产阶段，将 150 ml 分生孢子悬浮液（24.33 × 107 孢子/ml）在用 4% 葡萄糖和 1.5% 酵母提取物修正的 SDA 液体培养基中培养 10 天。然后使用 SDA 培养基接种一个容器的水稻底物。分生孢子悬浮液与基质的最佳比例 (v/v) 为 20-25%。从水稻底物收获的孢子粉对蓟马的毒力与在 SDA 固体培养基上生产的相同。在豇豆 (Vigna sinensis Endl) 田间施用蓟马 M. usitatus 孢子粉的控制水平与化学农药多杀菌素的控制水平相似。优化后的水稻底物体系可用于B. bassiana孢子的规模化生产，可用于蓟马的田间防治。usitatus 在豇豆 (Vigna sinensis Endl) 田间施用时与化学农药多杀菌素相似。优化后的水稻底物体系可用于B. bassiana孢子的规模化生产，可用于蓟马的田间防治。usitatus 在豇豆 (Vigna sinensis Endl) 田间施用时与化学农药多杀菌素相似。优化后的水稻底物体系可用于B. bassiana孢子的规模化生产，可用于蓟马的田间防治。