The two-phase olive mill waste is enormously produced in the Mediterranean area. This major waste is significantly rich in bioactive compounds that are highly valued by industry, such as phenolic and triterpenic compounds. Here, a thorough study of the most suitable solvent, extraction time and temperature for the large-volume, solid-liquid extraction of bioactive compounds has been made, in order to achieve maximum concentrations of the target compounds. Ultrasound effect has been considered. A deep characterization of the extracts by high-performance liquid chromatography coupled with electrospray-quadrupole-time of flight-mass spectrometry (LC-ESI-qToF-MS) has contributed to evaluate the effect of the operational parameters on the extraction performance. Forty-four compounds have been found and classified in their corresponding chemical families. At the optimum experimental conditions (EtOH 50% (v/v), 40 °C, ultrasound-assisted), more than 6.8 mg/g of bioactive content was recovered, and it was later purified by means of ultrafiltration. The membrane UP005 retained a significant percentage of the organic matter, whereas most of the bioactive compounds were recovered in the permeate. This contributed not only to revalorize this waste, but also to reduce its organic load and phytotoxicity, thus protecting the ecosystem of the final disposal zone of the residue.

两相橄榄磨坊废料在地中海地区大量生产。这种主要废物含有大量工业界高度重视的生物活性化合物，例如酚类和三萜类化合物。在此，对生物活性化合物的大体积固液萃取最合适的溶剂、萃取时间和温度进行了深入研究，以达到目标化合物的最大浓度。考虑了超声效应。通过高效液相色谱结合电喷雾四极杆飞行时间质谱 (LC-ESI-qToF-MS) 对提取物进行深入表征，有助于评估操作参数对提取性能的影响。已经发现了 44 种化合物，并将其归类到相应的化学家族中。在最佳实验条件下（EtOH 50% (v/v), 40 °C, 超声辅助），回收了超过 6.8 mg/g 的生物活性成分，随后通过超滤对其进行纯化。膜 UP005 保留了显着百分比的有机物，而大部分生物活性化合物在渗透液中被回收。这不仅有助于重新评估这些废物的价值，还有助于减少其有机负荷和植物毒性，从而保护残留物最终处置区的生态系统。膜 UP005 保留了显着百分比的有机物，而大部分生物活性化合物在渗透液中被回收。这不仅有助于重新评估这些废物的价值，还有助于减少其有机负荷和植物毒性，从而保护残留物最终处置区的生态系统。膜 UP005 保留了显着百分比的有机物，而大部分生物活性化合物在渗透液中被回收。这不仅有助于重新评估这些废物的价值，还有助于减少其有机负荷和植物毒性，从而保护残留物最终处置区的生态系统。