Twelve isolates of entomopathogenic fungi belonging to Metarhizium robertsii, M. pinghaense, M. brunneum, Beauveria bassiana, and Isaria fumosorosea were screened against tarnished plant bug. All isolates were pathogenic, causing mortality from 28.8 ± 3.4 to 96.3 ± 2.7%. The LT50 values ranged from 2.7 to 6.0 d while the LT90 values varied between 6.6 and 15.0 d. Metarhizium robertsii isolate CPD6 (will be under the trade name NoVil) was among the isolates that caused high mortality within shorter times and was selected for study on developmental stages and greenhouse trial. The third-, fourth-, and fifth-instar nymphs, and adults were inoculated with 106, 107, and 108 conidia per ml of NoVil. All the stages were susceptible to fungal infection. However, third and fourth instars were the most susceptible with no significant differences in mortality across the three concentrations. On the other hand, mortality was dose-dependent with fifth-instar nymph and adult stages. The LT50 and LT90 values were also dose-dependent, with higher concentrations having shorter lethal-time values as compared to the lower concentrations. In the greenhouse, pepper plants were sprayed with NoVil and chemical insecticide Flonicamid (as industrial standard), before releasing adult tarnished plant bug. Mortality of 37.3, 75.5, and 76.3% was recorded in the control, NoVil, and Flonicamid, respectively. This study has identified NoVil as a potential mycoinsecticide candidate for the control of tarnished plant bug under greenhouse conditions. Further field testing on juvenile and adults is needed to evaluate the potential for in-field control.

中文翻译：

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昆虫病原真菌分离物对失去光泽的植物盲蝽（半翅目：Miridae）的感染性

筛选了 12 株属于绿僵菌、M. pinghaense、M. brunneum、Beauveria bassiana 和 Isaria fumosorosea 的昆虫病原真菌，以对抗失去光泽的植物臭虫。所有分离物都是致病的，导致死亡率从 28.8 ± 3.4 到 96.3 ± 2.7%。LT50 值在 2.7 到 6.0 天之间，而 LT90 值在 6.6 到 15.0 天之间变化。Metarhizium robertsii 分离株 CPD6（商品名为 NoVil）是在较短的时间内导致高死亡率的分离株之一，并被选择用于发育阶段和温室试验的研究。三龄、四龄和五龄若虫和成虫每毫升 NoVil 接种 106、107 和 108 个分生孢子。所有阶段都容易受到真菌感染。然而，三龄和四龄最容易受到影响，三个浓度的死亡率没有显着差异。另一方面，死亡率在五龄若虫和成虫阶段呈剂量依赖性。LT50 和 LT90 值也是剂量依赖性的，与较低浓度相比，较高浓度具有较短的致死时间值。在温室中，辣椒植物被喷洒了 NoVil 和化学杀虫剂氟硝酰胺（作为工业标准），然后释放成虫失去光泽的植物虫子。对照组、NoVil 和氟尼卡胺的死亡率分别为 37.3、75.5 和 76.3%。这项研究已将 NoVil 确定为在温室条件下控制失去光泽的植物臭虫的潜在杀真菌剂候选物。