Study question Can the use of a microfluidic sperm selection device without centrifugation simplify the procedure without affecting fertilization rates and embryonic development after ICSI? Summary answer The microfluidic device can be used to select sperm in a simple procedure without reducing the fertilization or embryo development rate. What is known already In human ART, it is essential to process the semen and adjust the sperm sample according to the intended purpose, rather than using raw semen. Current sperm preparation methods at most IVF clinics include the density gradient centrifugation method, which utilizes the difference in density between the maturation stages of sperm, followed by washing using centrifugation. However, these methods require a lot of steps and the procedure is complicated. Recently, several non-centrifugal sperm processing devices have become available. One of them is a simple sperm conditioning method using a microfluidic device. Study design, size, duration This was a prospective study using sibling oocytes including 20 ART patients treated with 24 cycles and where there were 320 zygotes after ICSI. The duration of the study was 8 months (May 2020 to December 2020). Participants/materials, setting, methods For sperm preparation without centrifugation, a microfluidic device “ZyMōtⓇ Multi 850μL” was used. According to the labelled use, 850μL of semen was required with “ZyMōtⓇ” device (ZyMōt group), and the rest of the semen was processed according to routine laboratory procedure by monolayer density gradient centrifugation and washing by centrifugation (DGC group). Oocytes from the same patient were randomly divided into 2 batches and ICSI was performed using sperm treated with each method. Main results and the role of chance The 2PN formation rate in the ZyMōt group was 84.5% (142/168), which was not significantly different from 82.9% (126/152) in the DGC group. There was also no significant difference in 1PN formation rate (3.0% vs. 3.3%), multi PN formation rate (3.6% vs. 3.3%) and the non-fertilization rate (8.3% vs. 10.5%). The good quality embryo rate at Day 3 was 25.0% (31/124) in the ZyMōt group and 24.5% (27/110) in the DGC group, with no significant difference. The Day 5 blastocyst rate was 37.9% (47/124) in the ZyMōt group and 36.7% (40/109) in the DGC group, and the cumulative blastocyst rates by Day 7 were 54.0% (67/124) and 57.8% (63/109), respectively, with no significant difference. Limitations, reasons for caution This study was limited to samples with a motile sperm concentration of more over 1.0 × 106 in raw semen. Wider implications of the findings These results demonstrated that sperm processing using the microfluidic device without centrifugation does not affect the fertilization or blastocyst development rate after ICSI and that the sperm processing procedure can be simplified by using this device. Trial registration number not applicable

中文翻译：

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P-048 使用无需离心的微流控精子选择装置进行胞浆内精子注射 (ICSI) 后的受精率和胚胎发育

研究问题 使用无需离心的微流控精子选择装置能否在不影响 ICSI 后受精率和胚胎发育的情况下简化程序？总结答案 微流体装置可用于通过简单的程序选择精子，而不会降低受精或胚胎发育率。已知情况 在人类 ART 中，必须根据预期目的处理精液并调整精子样本，而不是使用生精液。大多数IVF诊所目前的精子制备方法包括密度梯度离心法，该方法利用精子成熟阶段之间的密度差异，然后使用离心洗涤。然而，这些方法需要很多步骤并且程序复杂。最近，几种非离心精子处理设备已经可用。其中之一是使用微流体装置的简单精子调理方法。研究设计、规模、持续时间 这是一项使用同胞卵母细胞的前瞻性研究，包括 20 名接受 24 个周期治疗的 ART 患者，ICSI 后有 320 个受精卵。研究时间为 8 个月（2020 年 5 月至 2020 年 12 月）。参与者/材料、设置、方法对于无需离心的精子制备，使用了微流体装置“ZyMōtⓇ Multi 850μL”。根据标示用途，使用“ZyMōtⓇ”装置（ZyMōt组）需要850μL精液，其余精液按常规实验室程序通过单层密度梯度离心和离心洗涤（DGC组）进行处理。来自同一患者的卵母细胞被随机分为 2 批，并使用每种方法处理的精子进行 ICSI。主要结果和机会的作用 ZyMōt 组的 2PN 形成率为 84.5%（142/168），与 DGC 组的 82.9%（126/152）没有显着差异。1PN形成率（3.0% vs. 3.3%）、多PN形成率（3.6% vs. 3.3%）和非受精率（8.3% vs. 10.5%）也没有显着差异。ZyMōt组第3天优质胚胎率为25.0%（31/124），DGC组为24.5%（27/110），差异无统计学意义。ZyMōt组第5天囊胚率为37.9%（47/124），DGC组为36.7%（40/109），第7天累计囊胚率为54.0%（67/124）和57.8%（ 63/109），没有显着差异。局限性，谨慎的理由 本研究仅限于原始精液中活动精子浓度超过 1.0 × 106 的样本。研究结果的更广泛意义 这些结果表明，在没有离心的情况下使用微流体装置处理精子不会影响 ICSI 后的受精或囊胚发育率，并且使用该装置可以简化精子处理程序。试用注册号不适用 研究结果的更广泛意义 这些结果表明，在没有离心的情况下使用微流体装置处理精子不会影响 ICSI 后的受精或囊胚发育率，并且使用该装置可以简化精子处理程序。试用注册号不适用 研究结果的更广泛意义 这些结果表明，在没有离心的情况下使用微流体装置处理精子不会影响 ICSI 后的受精或囊胚发育率，并且使用该装置可以简化精子处理程序。试用注册号不适用