Human adenoviruses (HAdVs) are a major cause of epidemic keratoconjunctivitis. We investigated the types of adenoviruses responsible for the recent epidemic of keratoconjunctivitis in Korea. From January to November 2019, 218 conjunctival swab samples were collected from patients clinically suspected as having adenoviral keratoconjunctivitis. Genotyping targeting of adenovirus capsid hexon genes was performed using PCR and sequencing. Of the 218 samples collected, 128 (58.7%) were positive for the adenovirus genes by PCR, and 126 samples were successfully genotyped. Adenovirus type 8 (HAdV-D8) was the most common type (67.5%), followed by HAdV-D64 (11.1%), HAdV-D37 (9.5%), HAdV-B3 (5.6%), HAdV-D53 (4.0%), HAdV-E4 (1.6%), and HAdV-D56 (0.8%). Adenoviral keratoconjunctivitis cases were the most frequent in July and August 2019, which were mainly caused by type 8. Phylogenetic analyses revealed little genetic distance among adenoviruses of the same type detected in our study. Our results provide basic data for further studies of adenoviral keratoconjunctivitis.

中文翻译：

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韩国腺病毒性角结膜炎的分子流行病学。

人类腺病毒 (HAdVs) 是流行性角结膜炎的主要原因。我们调查了导致韩国最近流行的角膜结膜炎的腺病毒类型。从 2019 年 1 月至 2019 年 11 月，从临床怀疑患有腺病毒性角结膜炎的患者中收集了 218 份结膜拭子样本。使用 PCR 和测序对腺病毒衣壳六邻体基因进行基因分型。采集的218份样本中，128份（58.7%）的腺病毒基因经PCR检测呈阳性，126份样本成功进行基因分型。腺病毒 8 型 (HAdV-D8) 是最常见的类型 (67.5%)，其次是 HAdV-D64 (11.1%)、HAdV-D37 (9.5%)、HAdV-B3 (5.6%)、HAdV-D53 (4.0%) )、HAdV-E4 (1.6%) 和 HAdV-D56 (0.8%)。腺病毒性角结膜炎病例在 2019 年 7 月和 8 月最为常见，这主要是由8型引起的。系统发育分析表明，在我们的研究中检测到的同类型腺病毒之间的遗传距离很小。我们的研究结果为腺病毒性角结膜炎的进一步研究提供了基础数据。