Carbapenemase-producing Aeromonas species are an emerging health threat. This study aimed to determine carbapenemase-mediated resistance among Aeromonas isolates from the Akaki river, Ethiopia during the dry and wet seasons in 2019–2020. Antimicrobial susceptibility to carbapenems and cephalosporins was determined and carbapenemase production was confirmed. Of 163 isolates, the majority were human pathogens Aeromonas caviae (62), Aeromonas hydrophila (33) and Aeromonas veronii (49). These isolates were resistant to carbapenem and cephalosporin antibiotics, with the highest resistance to cefotaxime 86 (59.7%), ertapenem 71 (49.3%) and imipenem 65 (45.1%). Resistance to carbapenem antibiotics varied between species, where most A. veronii 37 (75.5%) and A. hydrophila 28 (84.8%) were resistant to imipenem and all A.caviae were sensitive. A. veronii, A. caviae and A. hydrophila resistance to meropenem was 31 (63.3%), 3 (4.8%) and 19 (57.6%), respectively. Of isolates resistant to carbapenem, 82.1% A. hydrophila and 94.4% A. veronii were carbapenemase producers. Cephalosporin resistance also varied among the different species. The highest resistance to carbapenem antibiotics was in isolates collected during the wet season (p<0.05); however, it was not consistent across all classes of antibiotics tested. The rivers in megacities could be reservoirs of carbapenemase-producing Aeromonas spp.

产生碳青霉烯酶的气单胞菌属是一种新兴的健康威胁。本研究旨在确定2019-2020 年旱季和雨季期间埃塞俄比亚 Akaki 河气单胞菌分离株中碳青霉烯酶介导的耐药性。确定了对碳青霉烯类和头孢菌素的抗菌敏感性，并证实了碳青霉烯酶的产生。在 163 个分离株中，大多数是人类病原体Aeromonas caviae (62)、Aeromonas hydrophila (33) 和Aeromonas veronii(49)。这些分离株对碳青霉烯类和头孢菌素类抗生素耐药，对头孢噻肟86（59.7%）、厄他培南71（49.3%）和亚胺培南65（45.1%）的耐药性最高。对碳青霉烯类抗生素的耐药性因物种而异，其中大多数A. veronii 37 (75.5%) 和A. hydrophila 28 (84.8%) 对亚胺培南耐药，所有A. caviae都敏感。A. veronii、A . caviae和A.hydrophila对美罗培南的耐药性分别为 31 (63.3%)、3 (4.8%) 和 19 (57.6%)。在对碳青霉烯类耐药的菌株中，82.1% A. hydrophila和 94.4% A. veronii是碳青霉烯酶的生产者。头孢菌素的耐药性在不同物种之间也存在差异。对碳青霉烯类抗生素的耐药性最高的是雨季收集的菌株（p <0.05）；然而，在所有测试的抗生素类别中并不一致。特大城市的河流可能是产碳青霉烯酶气单胞菌属的水库。