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Gas exchange analysers exhibit large measurement error driven by internal thermal gradients.
New Phytologist ( IF 9.4 ) Pub Date : 2022-07-30 , DOI: 10.1111/nph.18347
Josef C Garen 1 , Haley A Branch 1 , Isaac Borrego 1 , Benjamin Blonder 2 , Joseph R Stinziano 3 , Sean T Michaletz 1
Affiliation  

Portable gas exchange analysers provide critical data for understanding plant-atmosphere carbon and water fluxes, and for parameterising Earth system models that forecast climate change effects and feedbacks. We characterised temperature measurement errors in the Li-Cor LI-6400XT and LI-6800, and estimated downstream errors in derived quantities, including stomatal conductance (gsw ) and leaf intercellular CO2 concentration (Ci ). The LI-6400XT exhibited air temperature errors (differences between reported air temperature and air temperature measured near the leaf) up to 7.2°C, leaf temperature errors up to 5.3°C, and relative errors in gsw and Ci that increased as temperatures departed from ambient. This caused errors in leaf-to-air temperature relationships, assimilation-temperature curves and CO2 response curves. Temperature dependencies of maximum Rubisco carboxylation rate (Vcmax ) and maximum RuBP regeneration rate (Jmax ) showed errors of 12% and 35%, respectively. These errors are likely to be idiosyncratic and may differ among machines and environmental conditions. The LI-6800 exhibited much smaller errors. Earth system model predictions may be erroneous, as much of their parametrisation data were measured on the LI-6400XT system, depending on the methods used. We make recommendations for minimising errors and correcting data in the LI-6400XT. We also recommend transitioning to the LI-6800 for future data collection.

中文翻译:

气体交换分析仪表现出由内部热梯度驱动的大测量误差。

便携式气体交换分析仪为了解植物-大气碳和水通量以及为预测气候变化影响和反馈的地球系统模型参数化提供关键数据。我们描述了 Li-Cor LI-6400XT 和 LI-6800 中的温度测量误差,并估计了衍生量的下游误差,包括气孔导度 (gsw) 和叶细胞间 CO2 浓度 (Ci)。LI-6400XT 表现出高达 7.2°C 的气温误差(报告的气温和在叶片附近测量的气温之间的差异),高达 5.3°C 的叶片温度误差,以及随着温度偏离而增加的 gsw 和 Ci 的相对误差周围。这导致叶片与空气温度关系、同化温度曲线和二氧化碳响应曲线出现误差。最大 Rubisco 羧化率 (Vcmax) 和最大 RuBP 再生率 (Jmax) 的温度依赖性分别显示 12% 和 35% 的误差。这些错误可能是特殊的,并且可能因机器和环境条件而异。LI-6800 的误差要小得多。地球系统模型的预测可能是错误的,因为它们的大部分参数化数据是在 LI-6400XT 系统上测量的,具体取决于所使用的方法。我们建议尽量减少错误并纠正 LI-6400XT 中的数据。我们还建议过渡到 LI-6800 以便将来收集数据。LI-6800 的误差要小得多。地球系统模型的预测可能是错误的,因为它们的大部分参数化数据是在 LI-6400XT 系统上测量的,具体取决于所使用的方法。我们建议尽量减少错误并纠正 LI-6400XT 中的数据。我们还建议过渡到 LI-6800 以便将来收集数据。LI-6800 的误差要小得多。地球系统模型的预测可能是错误的,因为它们的大部分参数化数据是在 LI-6400XT 系统上测量的,具体取决于所使用的方法。我们建议尽量减少错误并纠正 LI-6400XT 中的数据。我们还建议过渡到 LI-6800 以便将来收集数据。
更新日期:2022-06-28
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