There is mounting evidence that acaricides are among the most prevalent medicinal compounds in honey bee hive matrices worldwide. According to OCDE guideline No. 245 chronic lethal concentration of tau-fluvalinate (at concentrations ranging from 77.5 to 523.18 ppm), coumaphos (59.8 ppm) and dimethoate (0.7 ppm) were determined. The activity of the biomarkers acetylcholinesterase (AChE), carboxylesterase (CbE), glutathione S-transferase (GST), catalase (CAT) and malondialdehyde (MDA) was analysed and as they are implicated in neurotoxicity, biotransformation and antioxidant defences, these values were combined into an integrated biomarker response (IBR). There was enhanced AChE, CAT and GST activity in honey bees exposed to tau-fluvalinate, while dimethoate inhibited AChE activity. Both dimethoate and coumaphos inhibited CbE activity but they enhanced CAT activity and MDA formation. Our results highlight how these biomarkers may serve to reveal honey bee exposure to commonly used acaricides.

越来越多的证据表明，杀螨剂是全世界蜜蜂蜂巢基质中最普遍的药用化合物之一。根据 OCDE 指南第 245 号慢性致死浓度 tau-fluvalinate（浓度范围为 77.5 至 523.18 ppm）、香豆磷（59.8 ppm）和乐果（0.7 ppm）。生物标志物乙酰胆碱酯酶 (AChE)、羧酸酯酶 (CbE)、谷胱甘肽S的活性对转移酶 (GST)、过氧化氢酶 (CAT) 和丙二醛 (MDA) 进行了分析，由于它们与神经毒性、生物转化和抗氧化防御有关，因此将这些值组合成综合生物标志物反应 (IBR)。暴露于 tau-fluvalinate 的蜜蜂中 AChE、CAT 和 GST 活性增强，而乐果抑制 AChE 活性。乐果和香豆磷都抑制 CbE 活性，但它们增强了 CAT 活性和 MDA 的形成。我们的结果强调了这些生物标志物如何有助于揭示蜜蜂接触常用杀螨剂的情况。