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Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry
Journal of Analytical Methods in Chemistry ( IF 2.6 ) Pub Date : 2022-06-28 , DOI: 10.1155/2022/7015311
Chen Lan 1 , Bing Zhao 1 , Lu Yang 1 , Yusen Zhou 1 , Siyi Guo 1 , Xuebin Zhang 1 , Junli Zhang 1
Affiliation  

Nucleotide sugars, the activated forms of monosaccharides, are important intermediates of carbohydrate metabolism in all organisms. Here, we describe a method for the detection and quantification of UDP-glucose and UDP-galactose in maize in order to compare their metabolism in both wild-type and mutated plants. Triple quadrupole operating in a multiple reaction monitoring mode was used to quantify nucleotide sugars. The limits of detection for UDP-glucose and UDP-galactose were 0.50 and 0.70 ng·mL−1, respectively. The recoveries of the method ranged from 98.3% to 103.6% with the relative standard deviations less than 6.3%. To prove the applicability of this method, we analyzed several sets of maize extracts obtained from different cultivars grown under standardized greenhouse conditions. All the results demonstrated the suitability of the developed method to quantify UDP-glucose and UDP-galactose in maize extracts.

中文翻译:

亲水相互作用液相色谱-串联质谱法测定玉米中UDP-葡萄糖和UDP-半乳糖

核苷酸糖是单糖的活化形式,是所有生物体中碳水化合物代谢的重要中间体。在这里,我们描述了一种检测和量化玉米中 UDP-葡萄糖和 UDP-半乳糖的方法,以便比较它们在野生型和突变植物中的代谢。以多反应监测模式运行的三重四极杆用于量化核苷酸糖。UDP-葡萄糖和 UDP-半乳糖的检测限分别为 0.50 和 0.70 ng·mL -1, 分别。该方法的回收率在 98.3% 到 103.6% 之间,相对标准偏差小于 6.3%。为了证明该方法的适用性,我们分析了从标准化温室条件下生长的不同品种获得的几组玉米提取物。所有结果都证明了所开发的方法适用于量化玉米提取物中的 UDP-葡萄糖和 UDP-半乳糖。
更新日期:2022-06-28
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