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Multifunctional microfluidic chips for the single particle inductively coupled plasma mass spectrometry analysis of inorganic nanoparticles
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-06-28 , DOI: 10.1039/d2lc00377e
Gyula Kajner 1 , Albert Kéri 1 , Ádám Bélteki 1 , Sándor Valkai 2 , András Dér 2 , Zsolt Geretovszky 3 , Gábor Galbács 1
Affiliation  

This study aimed at exploiting the so far unexploited potential of carrying out on-line sample pretreatment steps on microfluidic chips for single particle inductively coupled plasma mass spectrometry (spICP-MS) measurements, and demonstrating their ability to practically facilitate most of the simpler tasks involved in the spICP-MS analysis of nanoparticles. For this purpose, polydimethylsiloxane microfluidic chips, capable of high-range dilution and sample injection were made by casting, using high-precision, 3D-printed molds. Optimization of their geometry and functions was done by running several hydrodynamic simulations and by gravimetric, fluorescence enhanced microscope imaging and solution-based ICP-MS experiments. On the optimized microfluidic chips, several experiments were done, demonstrating the benefits of the approach and these devices, such as the determination of nanoparticle concentration using only a few tens of microliters of sample, elimination of solute interferences by dilution, solution-based size calibration and characterisation of binary nanoparticles. Due to the unique design of the chips, they can be linked together to extend the dilution range of the system by more than a magnitude per chip. This feature was also demonstrated in applications requiring multiple-magnitude dilution rates, when two chips were sequentially coupled.

中文翻译:

用于无机纳米粒子单粒子电感耦合等离子体质谱分析的多功能微流控芯片

本研究旨在利用迄今为止尚未开发的在微流控芯片上进行在线样品预处理步骤以进行单粒子电感耦合等离子体质谱 (spICP-MS) 测量的潜力,并证明它们能够切实促进所涉及的大多数简单任务用于纳米颗粒的 spICP-MS 分析。为此,使用高精度 3D 打印模具通过铸造制造了能够进行高范围稀释和样品注入的聚二甲基硅氧烷微流控芯片。通过运行多个流体动力学模拟以及重量分析、荧光增强显微镜成像和基于溶液的 ICP-MS 实验来优化它们的几何形状和功能。在优化的微流控芯片上,做了几个实验,展示了该方法和这些设备的好处,例如仅使用几十微升样品测定纳米颗粒浓度、通过稀释消除溶质干扰、基于溶液的尺寸校准和二元纳米颗粒的表征。由于芯片的独特设计,它们可以连接在一起,以将系统的稀释范围扩大超过每个芯片一个数量级。当两个芯片顺序耦合时,此功能也在需要多倍稀释率的应用中得到证明。它们可以连接在一起,以将系统的稀释范围扩大超过每芯片一个数量级。当两个芯片顺序耦合时,此功能也在需要多倍稀释率的应用中得到证明。它们可以连接在一起,以将系统的稀释范围扩大超过每芯片一个数量级。当两个芯片顺序耦合时,此功能也在需要多倍稀释率的应用中得到证明。
更新日期:2022-06-28
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