Endolysins encoded by bacteriophages are considered bacteriostatic agents for the prevention of pathogenic bacteria proliferations. In this study, a possible endolysin from bacteriophage JN01, namely LysJN01, belonging to the lysozyme-like superfamily, was cloned, expressed and characterized. Meanwhile, the bactericidal activity of recombinant LysJN01 (rLysJN01) against E. coli O157:H7 was evaluated in Tris-HCl buffer and on lettuce. Recombinant LysJN01(rLysJN01) consists of a single domain with a molecular weight of about 20 kDa. The yield of rLysJN01 was about 20 mg/L rLysJN01 had a strong lytic activity against chloroform treated E. coli O157:H7. It combined with EDTA could also significantly lyse E. coli O157:H7. Afterward, rlysJN01 showed high enzymatic activity stability at temperature (4–95 ^OC) and pH (5.0–11.0). Moreover, it also revealed a broad spectrum of antimicrobial activity against Gram-negative pathogens, such as E. coli, Salmonella and Pseudomonas aeruginosa. Furthermore, the counts of E. coli O157:H7 on contaminated lettuce was significantly reduced by 91.6% and 97.8% after treatment with a combination of 60 μg/mL rLysJN01 and 0.5 mmol/L EDTA for 30 min and 60 min, respectively. All these results indicated that rLysJN01 is a potential and efficient bacteriostatic agent for biocontrol of E. coli O157:H7.

由噬菌体编码的内溶素被认为是用于防止病原菌增殖的抑菌剂。在这项研究中，克隆、表达和表征了一种可能来自噬菌体 JN01 的内溶素，即 LysJN01，它属于类溶菌酶超家族。同时，在 Tris-HCl 缓冲液和生菜上评估重组 LysJN01 (rLysJN01) 对大肠杆菌O157:H7 的杀菌活性。重组 LysJN01(rLysJN01) 由分子量约为 20 kDa 的单个结构域组成。rLysJN01 的产量约为 20 mg/L rLysJN01 对氯仿处理的大肠杆菌O157:H7 具有很强的裂解活性。它与 EDTA 结合还可以显着裂解大肠杆菌O157：H7。^{之后， rlysJN01}在温度（4-95摄氏度）和 pH 值（5.0-11.0）下表现出高酶活性稳定性。此外，它还揭示了对革兰氏阴性病原体如大肠杆菌、沙门氏菌和铜绿假单胞菌的广谱抗菌活性。此外，在用 60 μg/mL rLysJN01 和 0.5 mmol/L EDTA 组合处理 30 分钟和 60 分钟后，受污染生菜上的大肠杆菌O157:H7 数量分别显着降低了 91.6% 和 97.8%。所有这些结果表明，rLysJN01是一种潜在的高效抑菌剂，可用于大肠杆菌O157:H7的生物防治。