Lipidomics coverage improvement is essential for functional lipid and pathway construction. A powerful approach to discovering organism lipidome is to combine various data acquisitions, such as full scan mass spectrometry (full MS), data-dependent acquisition (DDA), and data-independent acquisition (DIA). Caenorhabditis elegans (C. elegans) is a useful model for discovering toxic-induced metabolism, high-throughput drug screening, and a variety of human disease pathways. To determine the lipidome of C. elegans and investigate lipid disruption from the molecular level to the system biology level, we used integrative data acquisition. The methyl-tert-butyl ether method was used to extract L4 stage C. elegans after exposure to triclosan (TCS), perfluorooctanoic acid, and nanopolystyrene (nPS). Full MS, DDA, and DIA integrations were performed to comprehensively profile the C. elegans lipidome by Q-Exactive Plus MS. All annotated lipids were then analyzed using lipid ontology and pathway analysis. We annotated up to 940 lipids from 20 lipid classes involved in various functions and pathways. The biological investigations revealed that when C. elegans were exposed to nPS, lipid droplets were disrupted, whereas plasma membrane-functionalized lipids were likely to be changed in the TCS treatment group. The nPS treatment caused a significant disruption in lipid storage. Triacylglycerol, glycerophospholipid, and ether class lipids were those primarily hindered by toxicants. Finally, toxicant exposure frequently involved numerous lipid-related pathways, including the phosphoinositide 3-kinase/protein kinase B pathway. In conclusion, an integrative data acquisition strategy was used to characterize the C. elegans lipidome, providing valuable biological insights into hypothesis generation and validation.

脂质组学覆盖率的提高对于功能性脂质和通路构建至关重要。发现有机体脂质组的一种有效方法是结合各种数据采集，例如全扫描质谱（full MS）、数据相关采集（DDA）和数据独立采集（DIA）。秀丽隐杆线虫( C. elegans ) 是一种有用的模型，可用于发现毒性诱导的代谢、高通量药物筛选和各种人类疾病途径。为了确定秀丽隐杆线虫的脂质组并研究从分子水平到系统生物学水平的脂质破坏，我们使用了综合数据采集。甲基叔丁基醚法用于提取L4阶段秀丽隐杆线虫在接触三氯生 (TCS)、全氟辛酸和纳米聚苯乙烯 (nPS) 后。通过 Q-Exactive Plus MS进行完整的 MS、DDA 和 DIA 集成以全面分析秀丽隐杆线虫脂质组。然后使用脂质本体和通路分析分析所有注释的脂质。我们注释了来自 20 个脂质类别的多达 940 种脂质，这些脂质涉及各种功能和途径。生物学研究表明，当C. elegans暴露于 nPS，脂滴被破坏，而 TCS 治疗组中质膜功能化的脂质可能会发生变化。nPS 处理导致脂质储存显着中断。三酰基甘油、甘油磷脂和醚类脂质是主要受毒物阻碍的脂质。最后，毒物暴露经常涉及许多与脂质相关的途径，包括磷酸肌醇 3-激酶/蛋白激酶 B 途径。总之，使用综合数据采集策略来表征秀丽隐杆线虫脂质组，为假设的生成和验证提供有价值的生物学见解。