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Stress responses in captive Crocodylus moreletii associated with metal exposure
Environmental Pollution ( IF 8.9 ) Pub Date : 2022-06-25 , DOI: 10.1016/j.envpol.2022.119685
A G Romero-Calderón 1 , T Alvarez-Legorreta 1 , J Rendón von Osten 2 , M González-Jáuregui 3 , J R Cedeño-Vázquez 4
Affiliation  

Environmental pollution by metals has repercussions on wildlife health. It is known that some metals can have an influence on the neuroendocrine stress response, and at the same time, metals have pro-oxidant effects that can overwhelm the antioxidant system and cause oxidative stress. This study evaluates the association of metals with neuroendocrine stress activity and biomarkers of oxidative stress in 42 captive female Morelet's crocodiles (Crocodylus moreletii). We measured five metals of ecotoxicological importance (Hg, Cd, Pb, Cu and Zn), and three biomarkers of the oxidative stress response in the liver: glutathione (GSH) and glutathione disulfide (GSSG) as markers for antioxidant system and thiobarbituric acid reactive substances (TBARS) for oxidative damage. We also measured one biomarker of the neuroendocrine response to stress: corticosterone (B) in blood plasma. The mean ± SD concentrations of metals in the liver expressed in μg/g (dw) were: Cd: 0.004 ± 0.003, Hg: 0.014 ± 0.019, Cu: 0.017 ± 0.013, Zn: 0.043 ± 0.035, Pb: 0.16 ± 0.256. The mean ± SD of GSH was 0.42 ± 0.35 nmol/mg protein, the mean ± SD of GSSG was 0.24 ± 0.20 nmol/mg protein, the mean ± SD concentrations of TBARS were 0.36 ± 0.21 nmol/mg protein, and the mean ± SD of B was 393.57 ± 405.14 pg/mL. Hg presented a significant negative relationship with corticosterone. Cd had a negative relationship with both GSH and GSSG; meanwhile, Zn showed a negative relationship with TBARS levels, could be a protective element against hepatic oxidative damage. Finally, B had negative relationship with oxidative damage. The connection found between Hg and the neuroendocrine stress response, as well as the correlations of Cd and Zn with oxidative damage and antioxidant activity should be studied further, given their toxicological importance and implications for the conservation of C. moreletii and other crocodilians.



中文翻译:

与金属暴露相关的圈养鳄鱼的应激反应

金属造成的环境污染会对野生动物的健康产生影响。众所周知,一些金属会对神经内分泌应激反应产生影响,同时,金属具有促氧化作用,可以压倒抗氧化系统并引起氧化应激。本研究评估了 42 条圈养雌性莫雷特鳄鱼 ( Crocodylus moreletii ) 中金属与神经内分泌应激活性和氧化应激生物标志物的关联。)。我们测量了五种具有生态毒理学重要性的金属(汞、镉、铅、铜和锌),以及肝脏中氧化应激反应的三种生物标志物:谷胱甘肽 (GSH) 和谷胱甘肽二硫化物 (GSSG) 作为抗氧化系统和硫代巴比妥酸反应性的标志物物质 (TBARS) 用于氧化损伤。我们还测量了一种神经内分泌对压力反应的生物标志物:血浆中的皮质酮 (B)。以 μg/g (dw) 表示的肝脏中金属的平均 ± SD 浓度为:Cd:0.004 ± 0.003,Hg:0.014 ± 0.019,Cu:0.017 ± 0.013,Zn:0.043 ± 0.035,Pb:0.16 ± 0.256。GSH 的平均值 ± SD 为 0.42 ± 0.35 nmol/mg 蛋白,GSSG 的平均值 ± SD 为 0.24 ± 0.20 nmol/mg 蛋白,TBARS 的平均值 ± SD 浓度为 0.36 ± 0.21 nmol/mg 蛋白,平均值 ± B 的 SD 为 393.57 ± 405.14 pg/mL。汞与皮质酮呈显着负相关。Cd与GSH和GSSG均呈负相关;同时,Zn与TBARS水平呈负相关,可能是肝脏氧化损伤的保护元素。最后,B与氧化损伤呈负相关。鉴于汞和锌的毒理学重要性和对保护水银的意义,应进一步研究汞与神经内分泌应激反应之间的联系,以及镉和锌与氧化损伤和抗氧化活性的相关性。C. moreletii和其他鳄鱼。

更新日期:2022-06-25
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