Herein, a novel region recognition of precursor microRNA (Pre-miRNA) based on hyperbranched hybrid chain reaction (HB-HCR) amplification was constructed to effectively eliminate the interference of Pre-miRNA to the mature microRNA (miRNA) by establishing the linear mapping relation between the two fluorescence signals produced by the miRNA sequence in the Pre-miRNA and Pre-miRNA residues to first realize simultaneous sensitive detection of Pre-miRNA and miRNA as well as highly sensitive imaging of intracellular Pre-miRNA and miRNA, which solves one main challenge of in vitro tumor disease diagnostics: inaccurate detection of tumor-induced miRNA changes. Impressively, this strategy easily distinguishes cancer cells from normal cells and DNA-damaged cells by the difference in miRNA and Pre-miRNA expression, which provides an innovative approach for accurate clinical diagnosis of cancer and precise treatment of prognosis.

中文翻译：

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区分癌细胞和 DNA 损伤细胞：基于超支化混合链反应扩增的 Pre-miRNA 区域识别，用于 miRNA 和 Pre-miRNA 的同时灵敏检测和成像

本文构建了一种基于超支化杂交链反应（HB-HCR）扩增的前体微小RNA（Pre-miRNA）区域识别方法，通过建立线性映射关系，有效消除Pre-miRNA对成熟微小RNA（miRNA）的干扰。 Pre-miRNA 和 Pre-miRNA 残基中 miRNA 序列产生的两种荧光信号之间，首次实现 Pre-miRNA 和 miRNA 的同时灵敏检测以及细胞内 Pre-miRNA 和 miRNA 的高灵敏成像，解决了一个主要问题。体外挑战肿瘤疾病诊断：对肿瘤诱导的 miRNA 变化的不准确检测。令人印象深刻的是，该策略通过miRNA和Pre-miRNA表达的差异，很容易区分癌细胞与正常细胞和DNA损伤细胞，为临床准确诊断癌症和精准治疗预后提供了一种创新方法。