With the rapid spread and multigeneration variation of coronavirus, rapid drug development has become imperative. A major obstacle to addressing this issue is adequately constructing the cell membrane at the molecular level, which enables in vitro observation of the cell response to virus and drug molecules quantitatively, shortening the drug experiment cycle. Herein, we propose a rapid and label-free supported lipid bilayer-based lab-on-a-chip biosensor for the screening of effective inhibition drugs. An extended gate electrode was prepared and functionalized by an angiotensin-converting enzyme II (ACE2) receptor-incorporated supported lipid bilayer (SLB). Such an integrated system can convert the interactions of targets and membrane receptors into real-time charge signals. The platform can simulate the cell membrane microenvironment in vitro and accurately capture the interaction signal between the target and the cell membrane with minimized interference, thus observing the drug action pathway quantitatively and realizing drug screening effectively. Due to these label-free, low-cost, convenient, and integrated advantages, it is a suitable candidate method for the rapid drug screening for the early treatment and prevention of worldwide spread of coronavirus.

中文翻译：

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支持的基于脂质双层的芯片实验室生物传感器，用于快速电筛选冠状病毒药物

随着冠状病毒的快速传播和多代变异，快速药物开发已势在必行。解决这一问题的一个主要障碍是在分子水平上充分构建细胞膜，从而能够在体外定量观察细胞对病毒和药物分子的反应，从而缩短药物实验周期。在此，我们提出了一种快速且无标记的基于脂质双层的芯片实验室生物传感器，用于筛选有效的抑制药物。通过掺入血管紧张素转换酶 II (ACE2) 受体的支持脂质双层 (SLB) 制备和功能化扩展栅电极。这样的集成系统可以将目标和膜受体的相互作用转化为实时电荷信号。该平台可以模拟体外细胞膜微环境，以最小的干扰准确捕获靶标与细胞膜之间的相互作用信号，从而定量观察药物作用途径，有效实现药物筛选。由于具有无标记、低成本、方便、集成的优点，它是快速药物筛选的合适候选方法，用于早期治疗和预防冠状病毒的全球传播。