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Label-free hairpin probe for the rapid detection of Hg(II) based on T-Hg(II)-T
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2022-06-23 , DOI: 10.1016/j.aca.2022.340113
Xiaochuan Jin 1 , Ting Sun 1 , Ziyi Wu 1 , Deyong Wang 1 , Fan Hu 1 , Jiaxuan Xu 1 , Xian Li 1 , Jieqiong Qiu 1
Affiliation  

A simple thymine-rich mercury-specific oligonucleotide (G9T24C9) was designed to quickly detect Hg(Ⅱ) via thymine-Hg(II)-thymine (T-Hg(Ⅱ)-T) coordination chemistry by using 4′,6-diamidinyl-2-phenylindole (DAPI). When the stable GC-paired stem of the DNA hairpin occurred, DPAI could intercalate into the T-Hg(Ⅱ)-T base pairs as a fluorescent recognizer. As a result, the hairpin structure was able to promote the rapid formation of T-Hg(Ⅱ)-T mismatches in the presence of Hg(Ⅱ), trigger DAPI to recognize T-Hg(Ⅱ)-T as well as TA/AT base pairs and restore fluorescence; moreover, fluorescence increases were not observed when Hg(Ⅱ) was not introduced. This method represents a simple strategy to detect Hg(Ⅱ). Taking advantage of the hairpin structure, the fluorescence intensity of the G9T24C9 hairpin probe was positively correlated with the concentration of Hg(Ⅱ) from 2.87 to 1400 nM (R2 = 0.9968), and the limit of detection (3σ) was as low as 2.87 nM. Furthermore, this probe had high selectivity for Hg(Ⅱ) detection. The probe was applied to real samples of pond water for the detection of Hg(Ⅱ), and a recovery rate from 95.9% to 104.4% was obtained.



中文翻译:

基于T-Hg(II)-T快速检测Hg(II)的无标记发夹探针

设计了一种简单的富含胸腺嘧啶的汞特异性寡核苷酸 (G9T24C9),利用 4',6-二脒基通过胸腺嘧啶-Hg(II)-胸腺嘧啶 (T-Hg(Ⅱ)-T) 配位化学快速检测 Hg(Ⅱ) -2-苯基吲哚(DAPI)。当DNA发夹的稳定GC配对茎出现时,DPAI可以作为荧光识别剂插入到T-Hg(Ⅱ)-T碱基对中。因此,发夹结构能够在 Hg(Ⅱ) 存在的情况下促进 T-Hg(Ⅱ)-T 错配的快速形成,触发 DAPI 识别 T-Hg(Ⅱ)-T 以及 TA/ AT碱基对并恢复荧光;此外,当不引入Hg(Ⅱ)时,没有观察到荧光增加。该方法代表了一种检测 Hg(Ⅱ) 的简单策略。利用发夹结构,2  = 0.9968),检测限 (3σ) 低至 2.87 nM。此外,该探针对Hg(Ⅱ)的检测具有较高的选择性。将该探针应用于池塘水真实样品中Hg(Ⅱ)的检测,回收率在95.9%~104.4%之间。

更新日期:2022-06-23
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