A fast, simple and environmentally friendly method for the digestion of solid samples of Gentiana rigescens Franch. ex Hemsl. (G. rigescens) based on deep eutectic solvent (DES) was established, and inductively coupled plasma-optical emission spectrometry (ICP-OES) was used to quantify Ni, Zn, Co, Cr and Cu in DES digestion solution. Under optimized conditions, limits of detection (LOD) and limits of quantification (LOQ) ranged from 0.4 to 2.5 and 1.2–8.2 μg g^-1, respectively. The intra-day and inter-day precision were carried out by relative standard deviation (RSD) ranged from 0.8% to 4.9% and 0.6%–1.4%, and the spiked recoveries ranged from 90.6% to 111.9%, respectively. The results of quantitative analysis by the developed method were in good agreement with those of microwave digestion. At the same time, the contents of the metal ions in the G. rigescens decoction liquid and freeze-dried powder solution of the decoction liquid of G. rigescens (crude extract) were determined by ICP-OES. The results showed that the contents of Ni, Zn, Co and Cu in the decoction liquid and crude extract were significantly lower than the contents in DES digestion liquid, and Cr was not detected. Then, the oxidative damage model of LO2 cells induced by H₂O₂ was established. The protective effect of crude extract of G. rigescens on damaged liver cells and the effect of metal ions with the same contents of decoction liquid on liver protection of G. rigescens were investigated for the first time. The results show that H₂O₂ could induce the decrease of LO2 cell viability and damage the cell morphology. GPS and crude extract could inhibit H₂O₂-induced liver injury by adjusting the antioxidant enzyme system. Metal ions corresponding to the content of the decoction liquid may weaken the hepatoprotective effect of GPS or crude extract by regulating the antioxidant enzyme system.

一种快速、简单和环保的方法，用于消解龙胆草固体样品。前 Hemsl。建立了基于深共晶溶剂（DES）的（G. rigescens），采用电感耦合等离子体发射光谱法（ICP-OES）对DES消解液中的Ni、Zn、Co、Cr、Cu进行定量分析。在优化条件下，检测限 (LOD) 和定量限 (LOQ) 范围为 0.4 到 2.5 和 1.2-8.2 μg g ^{- 1}， 分别。日内和日间精密度通过相对标准偏差 (RSD) 进行，范围为 0.8% 至 4.9% 和 0.6% 至 1.4%，加标回收率范围分别为 90.6% 至 111.9%。所开发方法的定量分析结果与微波消解的结果吻合较好。同时，采用ICP-OES法测定了毛蕊花煎剂液和毛蕊花煎剂（粗提物）冻干粉溶液中金属离子的含量。结果表明，煎煮液和粗提液中Ni、Zn、Co和Cu的含量明显低于DES消解液中的含量，未检出Cr。然后，H诱导的LO2细胞氧化损伤模型₂ O ₂成立。首次研究了牛蒡粗提物对受损肝细胞的保护作用，以及等量煎液中金属离子对牛蒡的保肝作用。结果表明，H ₂ O ₂可诱导LO2细胞活力下降，破坏细胞形态。GPS和粗提物可以通过调节抗氧化酶系统来抑制H ₂ O ₂引起的肝损伤。与煎剂液含量相对应的金属离子可能通过调节抗氧化酶系统而减弱GPS或粗提物的保肝作用。