The inhibition effect of amiloride on alamethicin ion channels was studied in a model zwitterionic floating bilayer lipid membrane (fBLM). The EIS studies indicated that amiloride prevents the transport of ions through the alamethicin channels leading to an overall increase in membrane resistance. The PM-IRRAS data demonstrated that amiloride has no influence on the secondary structure of alamethicin but restricts the insertion of the peptides into the bilayer and blocks ion transport through preformed alamethicin channels. The effect of amiloride on ion channel formation in the floating bilayer formed by a zwitterionic lipid was compared to those of previous studies involving negatively charged fBLMs and tethered zwitterionic lipid bilayers. The findings from these studies show that the effects of amiloride on ion channel formation strongly depend on the mobility and charge of the membrane lipids.

中文翻译：

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脂质组成对阿米洛利对负载型磷脂双分子层阿拉米辛离子通道抑制机制的影响

在模型两性离子浮动双层脂质膜（fBLM）中研究了阿米洛利对阿拉米辛离子通道的抑制作用。EIS 研究表明，阿米洛利可阻止离子通过 alamethicin 通道传输，从而导致膜电阻总体增加。PM-IRRAS 数据表明，阿米洛利对 alamethicin 的二级结构没有影响，但会限制肽插入双层并阻止离子通过预先形成的 alamethicin 通道传输。将阿米洛利对由两性离子脂质形成的浮动双层中离子通道形成的影响与先前涉及带负电荷的 fBLM 和束缚两性离子脂质双层的研究进行了比较。