Enzymes are versatile catalysts with high potential in various applications, and much attention has been paid to the stability improvement of native enzymes and activity modulation. Encapsulation in metal–organic frameworks (MOFs) as an efficient strategy for protecting fragile native enzymes while modulating the activity of enzymes remotely, which is practically demanded, has rarely been explored in MOF-encapsulated enzymes. Herein, Ti₃C₂ nanosheets exhibiting photothermal effect and biocompatibility were encapsulated in Cyt c-embedded ZIF-8 to tailor the enzymatic activity remotely by near-infrared (NIR) irradiation for the first time. By exposure to NIR light, the temperature of an aqueous solution containing Ti₃C₂/Cyt c@ZIF-8 increases obviously (up to 15 °C), while that of Cyt c@ZIF-8 shows no change. The enzymatic activity in the composites with a certain amount of nanosheets increases, which is attributed to the created defect and transformed microenvironment caused by the introduction of nanosheets. Importantly, the enzymatic activity in ZIF-8 can be further enhanced up to 150% under NIR light irradiation, and this enhancement can be modulated flexibly by varying laser power density. Our investigations indicate that Ti₃C₂ nanosheets are promising candidates for modulating the activity of encapsulated enzymes remotely.

中文翻译：

---

利用 Ti3C2 纳米片的光热效应调节金属有机框架中酶的活性

酶是多功能催化剂，在各种应用中具有很高的潜力，天然酶的稳定性提高和活性调节受到了广泛关注。金属有机框架 (MOF) 中的封装作为一种有效的策略，可以保护脆弱的天然酶，同时远程调节酶的活性，这是实际需要的，但在 MOF 封装的酶中很少被探索。在此，具有光热效应和生物相容性的 Ti ₃ C ₂纳米片首次被封装在嵌入 Cyt c的 ZIF-8 中，以通过近红外 (NIR) 照射远程定制酶活性。_{通过暴露在近红外光下，含有 Ti 3} C ₂的水溶液的温度/Cyt c @ZIF-8 明显升高（最高 15 °C），而 Cyt c @ZIF-8 没有变化。具有一定量纳米片的复合材料中的酶活性增加，这归因于纳米片的引入造成的缺陷和微环境的改变。重要的是，在近红外光照射下，ZIF-8 中的酶活性可以进一步增强高达 150%，并且这种增强可以通过改变激光功率密度来灵活调节。我们的研究表明，Ti ₃ C ₂纳米片是用于远程调节封装酶活性的有希望的候选者。