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Additional principles that govern the release of pre-ribosomes from the nucleolus into the nucleoplasm in yeast
Nucleic Acids Research ( IF 14.9 ) Pub Date : 2022-06-23 , DOI: 10.1093/nar/gkac430
Amber J LaPeruta 1 , Jelena Micic 1 , John L Woolford 1
Affiliation  

During eukaryotic ribosome biogenesis, pre-ribosomes travel from the nucleolus, where assembly is initiated, to the nucleoplasm and then are exported to the cytoplasm, where assembly concludes. Although nuclear export of pre-ribosomes has been extensively investigated, the release of pre-ribosomes from the nucleolus is an understudied phenomenon. Initial data indicate that unfolded rRNA interacts in trans with nucleolar components and that, when rRNA folds due to ribosomal protein (RP) binding, the number of trans interactions drops below the threshold necessary for nucleolar retention. To validate and expand on this idea, we performed a bioinformatic analysis of the protein components of the Saccharomyces cerevisiae ribosome assembly pathway. We found that ribosome biogenesis factors (RiBi factors) contain significantly more predicted trans interacting regions than RPs. We also analyzed cryo-EM structures of ribosome assembly intermediates to determine how nucleolar pre-ribosomes differ from post-nucleolar pre-ribosomes, specifically the capacity of RPs, RiBi factors, and rRNA components to interact in trans. We observed a significant decrease in the theoretical trans-interacting capability of pre-ribosomes between nucleolar and post-nucleolar stages of assembly due to the release of RiBi factors from particles and the folding of rRNA. Here, we provide a mechanism for the release of pre-ribosomes from the nucleolus.

中文翻译:

控制酵母中前核糖体从核仁释放到核质的其他原则

在真核核糖体生物发生过程中,前核糖体从组装开始的核仁移动到核质,然后输出到细胞质,组装结束。尽管前核糖体的核输出已被广泛研究,但前核糖体从核仁的释放仍是一个尚未得到充分研究的现象。初始数据表明,未折叠的 rRNA 与核仁成分发生反式相互作用,并且当 rRNA 由于核糖体蛋白 (RP) 结合而折叠时,反式相互作用的数量降至核仁保留所需的阈值以下。为了验证和扩展这个想法,我们对酿酒酵母核糖体组装途径的蛋白质成分进行了生物信息学分析。我们发现核糖体生物发生因子(RiBi 因子)比 RP 包含更多的预测反式相互作用区域。我们还分析了核糖体组装中间体的冷冻电镜结构,以确定核仁前核糖体与核仁后前核糖体有何不同,特别是 RP、RiBi 因子和 rRNA 成分反式相互作用的能力。我们观察到,由于 RiBi 因子从颗粒中释放和 rRNA 折叠,核仁前核糖体和核仁后组装阶段之间理论上的反式相互作用能力显着下降。在这里,我们提供了一种从核仁释放前核糖体的机制。
更新日期:2022-06-23
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