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Assembly of Fluorescent Polymer Nanoparticles Using Different Microfluidic Mixers
Langmuir ( IF 3.9 ) Pub Date : 2022-06-22 , DOI: 10.1021/acs.langmuir.2c00534
Huaiyou Chen 1 , Ali Emre Celik 1 , Angela Mutschler 2 , Antoine Combes 1 , Anne Runser 1 , Andrey S Klymchenko 1 , Sébastien Lecommandoux 2 , Christophe A Serra 3 , Andreas Reisch 1
Affiliation  

Nanoprecipitation is a facile and efficient approach to the assembly of loaded polymer nanoparticles (NPs) for applications in bioimaging and targeted drug delivery. Their successful use in clinics requires reproducible and scalable synthesis, for which microfluidics appears as an attractive technique. However, in the case of nanoprecipitation, particle formation depends strongly on mixing. Here, we compare 5 different types of microfluidic mixers with respect to the formation and properties of poly(d-l-lactide-co-glycolide) (PLGA) and poly(methyl methacrylate) NPs loaded with a fluorescent dye salt: a cross-shaped mixer, a multilamination mixer, a split and recombine mixer, two herringbone mixers, and two impact jet mixers. Size and fluorescence properties of the NPs obtained with these mixers are evaluated. All mixers, except the cross-shaped one, yield NPs at least as small and fluorescent as those obtained manually. Notably in the case of impact jet mixers operated at high flow speeds, the size of the NPs could be strongly reduced from >50 nm down to <20 nm. Surprisingly, the fluorescence quantum yield of NPs obtained with these mixers also depends strongly on the flow speed, increasing, in the case of PLGA, from 30 to >70%. These results show the importance of precisely controlling the assembly conditions for loaded polymer NPs. The present work further provides guidance for choosing the optimal microfluidic setup for production of nanomaterials for biomedical applications.

中文翻译:

使用不同的微流体混合器组装荧光聚合物纳米粒子

纳米沉淀是一种组装负载聚合物纳米粒子 (NP) 的简便有效的方法,可用于生物成像和靶向药物递送。它们在临床中的成功应用需要可重复和可扩展的合成,为此微流体似乎是一种有吸引力的技术。然而,在纳米沉淀的情况下,颗粒的形成很大程度上取决于混合。在这里,我们比较了 5 种不同类型的微流体混合器关于聚(d - l -lactide- co )的形成和性质。-乙交酯)(PLGA)和装有荧光染料盐的聚(甲基丙烯酸甲酯)纳米颗粒:十字形混合器、多层混合器、分离和重组混合器、两个人字形混合器和两个冲击喷射混合器。评估了使用这些混合器获得的 NP 的尺寸和荧光特性。所有混合器,除了十字形的混合器,产生的 NPs 至少与手动获得的那些一样小和荧光。值得注意的是,在冲击射流混合器以高流速运行的情况下,纳米颗粒的尺寸可以从 >50 nm 大幅减小到 <20 nm。令人惊讶的是,用这些混合器获得的 NPs 的荧光量子产率也很大程度上取决于流速,在 PLGA 的情况下,从 30% 增加到 >70%。这些结果表明精确控制负载聚合物纳米粒子的组装条件的重要性。
更新日期:2022-06-22
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