Purpose

Nitrogen (N) and sulphur (S) are essential for plant growth and development. Cysteine (Cys) and methionine (Met) are N- and S-containing amino acids in soils. However, it is unclear whether plants possess a strong ability to utilise N- and S-containing amino acids from the plant physiology perspective, and whether they can access amino acids when facing rapid microbial decomposition in the soil.

Methods

Wheat and oilseed rape were cultivated using a sterilised hydroponic solution in the laboratory and field conditions with ¹³C-, ¹⁴C-, ¹⁵N-, and ³⁵S-labelled Cys and Met.

Results

With sterilised hydroponic cultivation, wheat and oilseed rape possessed a greater ability for Cys and Met uptake than for SO₄²⁻ uptake, but these compounds did not support plant growth at high concentrations. The uptake rate of Cys and Met in oilseed rape was almost 20-fold higher than that in wheat, while the transportation ratio was even higher, indicating that oilseed rape not only possesses a great ability for S-containing amino acid uptake but also metabolises and transports them to the shoot quickly. A short-term labelling uptake test (6 h) in the field showed that 0.6–2.2% of total added Cys and Met were utilised by wheat and oilseed rape in the intact form owing to fierce competition from soil microorganisms.

Conclusions

Wheat and oilseed rape possess a great ability for Cys and Met uptake but can access limited amounts owing to rapid microbial decomposition in soil.

中文翻译：

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由于微生物的快速分解，植物可以在土壤中获取有限量的含氮和含硫氨基酸

目的

氮 (N) 和硫 (S) 对植物的生长和发育至关重要。半胱氨酸 (Cys) 和蛋氨酸 (Met) 是土壤中含 N 和 S 的氨基酸。然而，从植物生理学的角度来看，植物是否具有很强的利用含氮和硫氨基酸的能力，以及它们在土壤中面临快速微生物分解时是否能够获取氨基酸，尚不清楚。

方法

小麦和油菜是在实验室和田间条件下使用灭菌的水培溶液培养的，具有¹³ C-、¹⁴ C-、¹⁵ N-和³⁵ S-标记的 Cys 和 Met。

结果

通过灭菌水培栽培，小麦和油菜对 Cys 和 Met 的吸收能力比对 SO ₄ ^2-的吸收能力更强，但这些化合物在高浓度下不支持植物生长。油菜对 Cys 和 Met 的吸收率几乎是小麦的 20 倍，而转运率则更高，说明油菜不仅具有很强的含硫氨基酸吸收能力，而且还具有代谢和代谢的能力。将它们快速运送到拍摄地点。在田间进行的短期标记吸收试验（6 小时）表明，由于来自土壤微生物的激烈竞争，小麦和油菜以完整形式利用了总添加的 Cys 和 Met 的 0.6-2.2%。

结论

小麦和油菜具有很强的 Cys 和 Met 吸收能力，但由于土壤中微生物的快速分解，它们的吸收量有限。