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Multicolor Polystyrene Nanosensors for the Monitoring of Acidic, Neutral, and Basic pH Values and Cellular Uptake Studies
Analytical Chemistry ( IF 7.4 ) Pub Date : 2022-06-22 , DOI: 10.1021/acs.analchem.2c00944
Priyanka Srivastava 1 , Isabella Tavernaro 1 , Claudia Genger 2 , Pia Welker 2 , Oskar Hübner 1 , Ute Resch-Genger 1
Affiliation  

A first tricolor fluorescent pH nanosensor is presented, which was rationally designed from biocompatible carboxylated polystyrene nanoparticles and two analyte-responsive molecular fluorophores. Its fabrication involved particle staining with a blue-red-emissive dyad, consisting of a rhodamine moiety responsive to acidic pH values and a pH-inert quinoline fluorophore, followed by the covalent attachment of a fluorescein dye to the particle surface that signals neutral and basic pH values with a green fluorescence. These sensor particles change their fluorescence from blue to red and green, depending on the pH and excitation wavelength, and enable ratiometric pH measurements in the pH range of 3.0–9.0. The localization of the different sensor dyes in the particle core and at the particle surface was confirmed with fluorescence microscopy utilizing analogously prepared polystyrene microparticles. To show the application potential of these polystyrene-based multicolor sensor particles, fluorescence microscopy studies with a human A549 cell line were performed, which revealed the cellular uptake of the pH nanosensor and the differently colored emissions in different cell organelles, that is, compartments of the endosomal-lysosomal pathway. Our results demonstrate the underexplored potential of biocompatible polystyrene particles for multicolor and multianalyte sensing and bioimaging utilizing hydrophobic and/or hydrophilic stimuli-responsive luminophores.

中文翻译:

用于监测酸性、中性和碱性 pH 值和细胞吸收研究的多色聚苯乙烯纳米传感器

提出了第一个三色荧光 pH 纳米传感器,它是由生物相容的羧化聚苯乙烯纳米粒子和两种分析物响应分子荧光团合理设计的。它的制造涉及用蓝色-红色发射二元对颗粒染色,该二元由对酸性 pH 值响应的罗丹明部分和 pH 惰性喹啉荧光团组成,然后将荧光素染料共价连接到颗粒表面,发出中性和碱性信号pH 值带有绿色荧光。这些传感器颗粒根据 pH 值和激发波长将其荧光从蓝色变为红色和绿色,并能够在 3.0–9.0 的 pH 范围内进行比率 pH 测量。使用类似制备的聚苯乙烯微粒通过荧光显微镜确认不同传感器染料在颗粒核心和颗粒表面的定位。为了展示这些基于聚苯乙烯的多色传感器颗粒的应用潜力,对人类 A549 细胞系进行了荧光显微镜研究,揭示了 pH 纳米传感器的细胞摄取以及不同细胞器(即细胞器的隔室)中不同颜色的发射。内体-溶酶体途径。我们的结果证明了生物相容性聚苯乙烯颗粒在利用疏水性和/或亲水性刺激响应发光体进行多色和多分析物传感和生物成像方面的潜力尚未得到充分开发。为了展示这些基于聚苯乙烯的多色传感器颗粒的应用潜力,对人类 A549 细胞系进行了荧光显微镜研究,揭示了 pH 纳米传感器的细胞摄取以及不同细胞器(即细胞器的隔室)中不同颜色的发射。内体-溶酶体途径。我们的结果证明了生物相容性聚苯乙烯颗粒在利用疏水性和/或亲水性刺激响应发光体进行多色和多分析物传感和生物成像方面的潜力尚未得到充分开发。为了展示这些基于聚苯乙烯的多色传感器颗粒的应用潜力,对人类 A549 细胞系进行了荧光显微镜研究,揭示了 pH 纳米传感器的细胞摄取以及不同细胞器(即细胞器的隔室)中不同颜色的发射。内体-溶酶体途径。我们的结果证明了生物相容性聚苯乙烯颗粒在利用疏水性和/或亲水性刺激响应发光体进行多色和多分析物传感和生物成像方面的潜力尚未得到充分开发。内体-溶酶体途径的隔室。我们的结果证明了生物相容性聚苯乙烯颗粒在利用疏水性和/或亲水性刺激响应发光体进行多色和多分析物传感和生物成像方面的潜力尚未得到充分开发。内体-溶酶体途径的隔室。我们的结果证明了生物相容性聚苯乙烯颗粒在利用疏水性和/或亲水性刺激响应发光体进行多色和多分析物传感和生物成像方面的潜力尚未得到充分开发。
更新日期:2022-06-22
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