The black carpenter ant (Camponotus pennsylvanicus) is a pest species found widely throughout North America. From a single individual I used long-read nanopore sequencing to assemble a phased diploid genome of 306 Mb and 60X coverage, with quality assessed by a 97.0% BUSCO score, improving upon other ant assemblies. The mitochondrial genome reveals minor rearrangements from other ants. The reads also allowed assembly of parasitic and symbiont genomes. I include a complete Wolbachia bacterial assembly with a size of 1.2 Mb, as well as a commensal symbiont Blochmannia pennsylvanicus, at 791 kb. DNA methylation and hydroxymethylation were measured at base-pair resolution level from the same reads and confirmed extremely low levels seen in the Formicidae family. There was moderate heterozygosity, with 0.16% of bases being biallelic from the parental haplotypes. Protein prediction yielded 14 415 amino acid sequences with 95.8% BUSCO score and 86% matching to previously known proteins. All assemblies were derived from a single MinION flow cell generating 20 Gb of sequence for a cost of $1047 including consumable reagents. Adding fixed costs for equipment brings the total for an ant-sized genome to less than $5000. All analyses were performed in 1 week on a single desktop computer.

中文翻译：

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黑木匠蚂蚁 Camponotus pennsylvanicus 及其共生体的从头测序、二倍体组装和注释，由一个人使用纳米孔测序以 1000 美元的价格完成

黑木蚁 (Camponotus pennsylvanicus) 是一种广泛存在于北美的害虫。我使用长读长纳米孔测序从单个个体组装了一个 306 Mb 和 60X 覆盖的分相二倍体基因组，质量由 97.0% BUSCO 评分评估，改进了其他蚂蚁组装。线粒体基因组揭示了来自其他蚂蚁的微小重排。读数还允许组装寄生和共生基因组。我包括一个大小为 1.2 Mb 的完整 Wolbachia 细菌组装，以及一个 791 kb 的共生共生体 Blochmannia pennsylvanicus。DNA 甲基化和羟甲基化是在相同读数的碱基对分辨率水平上测量的，并证实了在蚁科中观察到的极低水平。存在中度杂合性，0. 16% 的碱基是来自亲本单倍型的双等位基因。蛋白质预测产生了 14415 个氨基酸序列，BUSCO 得分为 95.8%，与先前已知蛋白质的匹配率为 86%。所有组件均来自单个 MinION 流动池，生成 20 Gb 序列，成本为 1047 美元，包括耗材试剂。加上固定的设备成本，一个蚂蚁大小的基因组的总成本不到 5000 美元。所有分析均在 1 周内在一台台式计算机上完成。