Cotton is not only a raw material for the textile industry, but also an important strategic material. However, traditional breeding methods have narrowed the genetic diversity of current cotton cultivars. Abundant germplasm resources is a solid foundation for breeding, the use of chemical mutagenesis to create abundant mutation is of great significance to cotton breeding. This study used ethyl methanesulfonate (EMS) to mutagenize upland cotton material TM-1. First, we obtained a mutant library with abundant mutations and abundant phenotypes of the leaf, boll, locules numbers and plant architecture,. Second, based on the genome-wide analysis of the leaf and plant structure mutant., it showed that the library had a saturated genome mutations. Further GO and KEGG analysis showed SNPs were significantly enriched in DNA repair and response to hormone, non-homologous end joining (NHEJ) and homologous recombination (HR) pathways, which explained the mutated trait. In addition, we screened candidate gene (Gh_D05G364200) for crumpled leaf by BSA and transcriptome sequencing. Above all, the mutant library we obtained provides abundant resources for cotton functional genomics and breeding.

棉花不仅是纺织工业的原料，也是重要的战略物资。然而，传统的育种方法缩小了当前棉花品种的遗传多样性。丰富的种质资源是育种的坚实基础，利用化学诱变创造丰富的诱变对棉花育种具有重要意义。本研究使用甲磺酸乙酯(EMS) 对陆地棉材料 TM-1 进行诱变。首先，我们获得了一个突变体库，该突变体库具有丰富的突变和丰富的叶、铃、室等表型数字和植物结构，。其次，基于对叶片和植物结构突变体的全基因组分析，表明该文库具有饱和的基因组突变。进一步的 GO 和 KEGG 分析表明，SNP 在DNA 修复和对激素、非同源末端连接 (NHEJ) 和同源重组 (HR) 途径的反应中显着富集，这解释了突变的特征。此外，我们通过BSA和转录组测序筛选了皱叶的候选基因（Gh_D05G364200）。最重要的是，我们获得的突变体库为棉花功能基因组学和育种提供了丰富的资源。