Alkaline phosphatase (ALP), widely distributing in the human body, associates with many liver and bone diseases. Herein, we designed a reusable surface-enhanced Raman scattering (SERS) sensor via electrochemical self-assembly of Au nanoparticles (AuNPs) and zinc-based metal organic frameworks (Zn-MOFs) at anodic aluminum oxide (AAO) membrane for detecting ALP activity. The detection is based on the sensitive SERS response of the newly synthesized 2-mercaptoquinone (2-MBQ) modified on AuNPs surface towards ascorbic acid (AA) formed from the dephosphorylation of ascorbic acid 2-phosphate (AA2P) in the presence of ALP. Benefitting from the sieving function of Zn-MOFs and superiority of SERS, the developed sensor exhibited strong anti-interference performance and wide detection range from 1 to 300 U/L with a detection limit of 0.38 U/L, which allowed the direct detection of ALP activity in serum without any pretreatment. Besides, we regenerated the used sensor through the electrochemical oxidation of the reduced probe molecule 2-mercaptohydroquinone (2-MHQ). The regenerated sensor exhibited good maintenance of sensitive response to ALP, in favor of improving the usage efficiency and cutting the test cost. This work provides a promising platform for early diagnosis of ALP-related diseases and paves the way towards developing regenerable and pretreatment-free SERS sensors.

碱性磷酸酶（ALP）广泛分布于人体内，与许多肝脏和骨骼疾病有关。在此，我们设计了一种可重复使用的表面增强拉曼散射 (SERS) 传感器，通过在阳极氧化铝 (AAO) 膜上的金纳米粒子 (AuNPs) 和锌基金属有机骨架 (Zn-MOFs) 的电化学自组装来检测 ALP 活性. 该检测基于新合成的在 AuNPs 表面修饰的 2-巯基醌 (2-MBQ) 对 ALP 存在下抗坏血酸 2-磷酸 (AA2P) 去磷酸化形成的抗坏血酸 (AA) 的敏感 SERS 响应。受益于 Zn-MOFs 的筛分功能和 SERS 的优越性，开发的传感器具有很强的抗干扰性能，检测范围从 1 到 300 U/L，检测限为 0.38 U/L，这使得无需任何预处理即可直接检测血清中的 ALP 活性。此外，我们通过还原探针分子 2-巯基对苯二酚 (2-MHQ) 的电化学氧化来再生使用过的传感器。再生后的传感器对ALP的灵敏响应保持良好，有利于提高使用效率，降低测试成本。这项工作为 ALP 相关疾病的早期诊断提供了一个有前景的平台，并为开发可再生和免预处理的 SERS 传感器铺平了道路。有利于提高使用效率，降低测试成本。这项工作为 ALP 相关疾病的早期诊断提供了一个有前景的平台，并为开发可再生和免预处理的 SERS 传感器铺平了道路。有利于提高使用效率，降低测试成本。这项工作为 ALP 相关疾病的早期诊断提供了一个有前景的平台，并为开发可再生和免预处理的 SERS 传感器铺平了道路。