The abuse of amphetamine-type stimulants (ATS) has always been a worldwide problem and the law enforcement agencies are tasked and demanded with identifying them on-site rapidly and reliably in unknown samples. In the present study, an efficient electrochemical-surface plasmon resonance (EC-SPR) sensor, coupled with molecularly imprinted strategy, for adsorption and quantitative measurement of ATS in the human urine and serum samples was demonstrated. The molecular imprinted recognition system on SPR chip was synthesized by a convenient one-step electrochemical polymerization with 3,4-methylenedioxyphenethylamine (MDEA) as the template molecule and dopamine (DA) as the functional monomer. In particular, the molecularly imprinted process was regulated and controlled through synchronous measurement of the SPR signals. This proposed method showed lower detection limit with 57 nM and 59 nM for 3,4-methylenedioxeamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) in broad linearity, respectively and could be resistant to the interferences from various illicit drugs and other substances. Moreover, this EC-SPR sensor could be employed for detection the spiked MDMA in real urine and serum samples, significantly exhibiting attractive application prospect in forensic analysis.

苯丙胺类兴奋剂 (ATS) 的滥用一直是一个世界性问题，执法机构的任务和要求是在未知样本中快速可靠地现场识别它们。在本研究中，展示了一种高效的电化学表面等离子共振 (EC-SPR) 传感器，结合分子印迹策略，用于吸附和定量测量人尿液和血清样品中的 ATS。以3,4-亚甲基二氧苯乙胺（MDEA）为模板分子，多巴胺（DA）为功能单体，通过方便的一步电化学聚合合成了SPR芯片上的分子印迹识别系统。特别是，通过同步测量 SPR 信号来调节和控制分子印迹过程。该方法对 3,4-亚甲基二氧苯丙胺 (MDA) 和 3,4-亚甲基二氧甲基苯丙胺 (MDMA) 的检出限较低，分别为 57 nM 和 59 nM，具有较宽的线性，并且可以抵抗各种违禁药物和其他物质的干扰. 此外，该 EC-SPR 传感器可用于检测真实尿液和血清样品中的加标 MDMA，在法医分析中具有显着的应用前景。