Sugarcane bagasse (SCB), a widely available lignocellulosic by-product, could be potentially used in macrofungal bioconversion for high-value bioactive compounds. For macrofungus with poor lignin degradation capacity, its recalcitrant lignin structure blocked the release of fermentable sugars for cell growth. Herein, the main objective was to construct an effective delignification pretreatment strategy for SCB biotransformation and synthesis of bioactive substances by Agaricus sinodeliciosus var. Chaidam. Different pretreatments including chemical (alkali and Fenton oxidation) and ultrasound methods were used to intensify the delignification process. Physico-chemical characteristics of native and pretreated SCB were examined via XRD, XPS, FT-IR and ¹³C ssNMR to characterize crystallinity index, surface lignin and chemical structure. Lignocellulose-degrading enzymes were also used to assess the ability of the macrofungus to degrade SCB. By comparing morphological structure, chemical composition and fermentation characteristics, a suitable multiple sequential pretreatment method was finally identified. Our findings revealed that Fenton-ultrasound-alkali pre-treatment has an optimum delignification effect on SCB with never-uncovered excellent ability to detoxify harmful substances produced by Fenton oxidation. Under this pretreatment condition, the cellulose content was increased by 106.45%, whereas hemicelluloses and lignin were decreased by 48.34% and 74.15%, respectively. The macrofungal growth, intracellular polysaccharides, total phenols and terpenoids formation were 2.4, 1.46, 1.36 and 1.66 times higher than those of the untreated, respectively. Fermentation kinetic results showed outstanding fit and prediction of macrofungal growth and enzymatic activity secretion on above pretreated SCB. This study shed light on developing a novel pretreatment strategy for low-cost SCB lignocellulose transformation into high-value products.

甘蔗渣 (SCB) 是一种广泛可用的木质纤维素副产品，可潜在地用于高价值生物活性化合物的大型真菌生物转化。对于木质素降解能力较差的大型真菌，其顽固的木质素结构阻止了可发酵糖的释放以促进细胞生长。在此，主要目的是构建有效的脱木质素预处理策略，用于 SCB 生物转化和姬松茸var 合成生物活性物质。柴达姆。不同的预处理包括化学（碱和芬顿氧化）和超声方法用于强化脱木质素过程。^{通过 XRD、XPS、FT-IR 和13}检测天然和预处理 SCB 的物理化学特性C ssNMR 用于表征结晶度指数、表面木质素和化学结构。木质纤维素降解酶也用于评估大型真菌降解 SCB 的能力。通过比较形态结构、化学成分和发酵特性，最终确定了一种合适的多序贯预处理方法。我们的研究结果表明，芬顿-超声-碱预处理对 SCB 具有最佳的去木质作用，并且从未被发现具有出色的对芬顿氧化产生的有害物质的解毒能力。在这种预处理条件下，纤维素含量增加了106.45%，而半纤维素和木质素分别减少了48.34%和74.15%。大型真菌生长、细胞内多糖、总酚和萜类化合物的形成分别为2.4、1.46、1.36和1。分别是未处理组的 66 倍。发酵动力学结果显示出对上述预处理的 SCB 的大型真菌生长和酶活性分泌的出色拟合和预测。本研究揭示了开发一种将低成本 SCB 木质纤维素转化为高价值产品的新型预处理策略。