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Mapping of stem rot resistance in peanut indicates significant effect for plant architecture locus
Crop Science ( IF 2.3 ) Pub Date : 2022-06-18 , DOI: 10.1002/csc2.20803
Sarah Agmon 1 , Srinivas Kunta 1 , Mery Dafny Yelin 2 , Judit Moy 2 , Mwafaq Ibdah 3, 4 , Arye Harel 1 , Onn Rabinovitch 2 , Yael Levy 1 , Ran Hovav 1
Affiliation  

Stem rot, caused by the Sclerotium rolfsii, imposes severe yield losses in peanuts (Arachis hypogaea L.) worldwide. Breeding for resistance is challenging because not enough is known about mechanisms for resistance. The goals of this study were to (a) evaluate the field resistance of recombinant inbred lines derived from a resistant × susceptible cross, (b) associate quantitative loci (QTLs), and (c) reveal potential mechanisms for resistance. Lines were inspected during 3 yr in field conditions. Plants were manually inoculated and rated for three parameters: disease level, number of damaged plants/center, and disease power. Significant effects were found for the lines and the environment in all three parameters. Heritability and year-to-year correlations were highly significant, suggesting a consistent response of the lines to the stem rot pressure. Quantitative trait loci mapping was performed based on a previously constructed genetic map. Overall, 20 significant QTLs were found for the resistance parameters, concentrating in four locations on chromosomes A07, A03, B03, and B05. Out of the four loci, three were reported in previous studies with different genetic backgrounds suggesting a wide effect. The B05 QTL was the strongest, with the phenotypic variation explained of 11.6–21.7%. Interestingly, this QTL is colocalized with a previously identified major locus for branching habit trait. An additional field trial performed on 14 lines found a significant branching habit effect, wherein bunch-types lines were more resistant than spreading-types, suggesting that plant architecture can be a possible factor influencing the infection rate of S. rolfsii on the field level.

中文翻译:

花生茎腐病抗性图谱表明对植物结构位点有显着影响

由Sclerotium rolfsii引起的茎腐病对花生造成严重的产量损失(Arachis hypogaeaL.) 全世界。抗性育种具有挑战性,因为对抗性机制知之甚少。本研究的目标是 (a) 评估源自抗性×易感杂交的重组自交系的田间抗性,(b) 关联定量位点 (QTL),以及 (c) 揭示抗性的潜在机制。在 3 年的现场条件下对线路进行了检查。植物被手动接种并根据三个参数进行评级:疾病水平、受损植物/中心的数量和疾病能力。在所有三个参数中都发现了对线路和环境的显着影响。遗传力和年际相关性非常显着,表明品系对茎腐病压力的一致反应。基于先前构建的遗传图谱进行数量性状位点作图。总体而言,针对抗性参数发现了 20 个显着 QTL,集中在染色体 A07、A03、B03 和 B05 的四个位置。在这四个基因座中,三个基因座在之前的研究中被报道过,具有不同的遗传背景,表明具有广泛的影响。B05 QTL 最强,表型变异解释为 11.6–21.7%。有趣的是,该 QTL 与先前确定的分支习性特征的主要位点共定位。对 14 个品系进行的额外田间试验发现了显着的分枝习性效应,其中束型品系比传播型品系更具抗性,表明植物结构可能是影响感染率的一个可能因素 在这四个基因座中,三个基因座在之前的研究中被报道过,具有不同的遗传背景,表明具有广泛的影响。B05 QTL 最强,表型变异解释为 11.6–21.7%。有趣的是,该 QTL 与先前确定的分支习性特征的主要位点共定位。对 14 个品系进行的额外田间试验发现了显着的分枝习性效应,其中束型品系比传播型品系更具抗性,表明植物结构可能是影响感染率的一个可能因素 在这四个基因座中,三个基因座在之前的研究中被报道过,具有不同的遗传背景,表明具有广泛的影响。B05 QTL 最强,表型变异解释为 11.6–21.7%。有趣的是,该 QTL 与先前确定的分支习性特征的主要位点共定位。对 14 个品系进行的额外田间试验发现了显着的分枝习性效应,其中束型品系比传播型品系更具抗性,表明植物结构可能是影响感染率的一个可能因素 该 QTL 与先前确定的分支习性特征的主要位点共定位。对 14 个品系进行的额外田间试验发现了显着的分枝习性效应,其中束型品系比传播型品系更具抗性,表明植物结构可能是影响感染率的一个可能因素 该 QTL 与先前确定的分支习性特征的主要位点共定位。对 14 个品系进行的额外田间试验发现了显着的分枝习性效应,其中束型品系比传播型品系更具抗性,表明植物结构可能是影响感染率的一个可能因素S. rolfsii在田间水平。
更新日期:2022-06-18
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