Aflatoxin B₁, a secondary metabolite produced by fungi, is one of the most toxic mycotoxins that poses a major food security and public health threat worldwide. Effective sample pretreatment and high sensitivity detection techniques are urgently needed due to its trace amount in complex samples. Herein, an integrated detection strategy was developed by combining Mg/Zn-metal organic framework-74 modified Fe₃O₄ magnetic nanoparticles (Mg/Zn-MOF-74 @Fe₃O₄ MNPs)-based sample preparation and microchannel resistance biosensor for rapid and highly sensitive detection of aflatoxin B₁ in food samples. The synthesis and characterization of Mg/Zn-MOF-74 @Fe₃O₄ MNPs was reported, which exhibited efficient separation and enrichment capacity when exposed to complex grain samples. The competitive immunoassay-based microchannel resistance biosensor enabled specific and high-sensitive analysis of aflatoxin B₁ by using current as a readout, which caused by the blocking effect between the functionalized-polystyrene microspheres and microchannel. Under optimized conditions, this biosensor was capable to quantitatively analysis aflatoxin B₁ from 10 pg/mL to 20 ng/mL, and with a limit of detection of 4.75 pg/mL. This integrated detection strategy has been tested for the quantitative detection of aflatoxin B₁ in grain samples that is a potential protocol for food safety control and environmental monitoring.

黄曲霉毒素 B ₁是一种由真菌产生的次级代谢产物，是毒性最强的霉菌毒素之一，对全球粮食安全和公共卫生构成重大威胁。由于其在复杂样品中的痕量，迫切需要有效的样品预处理和高灵敏度检测技术。在此，基于 Mg/Zn-金属有机框架-74 修饰的 Fe ₃ O ₄磁性纳米粒子 (Mg/Zn-MOF-74 @Fe ₃ O ₄ MNPs) 的样品制备和微通道电阻生物传感器相结合，开发了一种集成检测策略。快速、高灵敏度地检测食品样品中的黄曲霉毒素 B ₁。Mg/Zn-MOF-74@Fe ₃ O的合成与表征报道了_{4种 MNP，它们在暴露于复杂的谷物样品时表现出有效的分离和富集能力。}基于竞争性免疫测定的微通道电阻生物传感器通过使用电流作为读数，实现了对黄曲霉毒素 B ₁的特异性和高灵敏度分析，这是由功能化聚苯乙烯微球和微通道之间的阻塞效应引起的。在优化条件下，该生物传感器能够定量分析10 pg/mL至20 ng/mL的黄曲霉素B _{1 ，检测限为4.75 pg/mL。}这种综合检测策略已经过测试，可用于粮食样品中黄曲霉毒素 B ₁的定量检测，这是食品安全控制和环境监测的潜在方案。