Buriti (Mauritia flexuosa L.) is a significant source of carotenoids, but these compounds have been extracted using laborious and low-effective methods. The present work evaluated the high-intensity ultrasound combined with a chemometric approach to developing an optimal extraction method of carotenoids from buriti pulp. The multivariate optimization was carried out through two steps. First, a simplex-lattice mixture design was used to optimize the extractor solution finding higher extraction yield (903 ± 21 µg g⁻¹) with the acetone:ethanol (75/25) mixture. After, sample mass (80 mg) and sonication time (30 min) were optimized applying central composite design (CCD) which provided a 14% improvement in the extraction method yield. So, the total carotenoid content (TCC) with optimal extraction conditions was 1026 ± 13 µg g⁻¹ which is almost twice the yield of methods known in the literature for buriti. The RP-HPLC-DAD analysis revealed that the carotenoids are gently extracted and β-carotene is the major compound in the extracts. To confirm the accuracy, buriti samples spiked with β-carotene standard and the developed method showed recovery >84% and precision <6.5%. Furthermore, the optimized ultrasound-assisted extraction (UAE) method was applied to other samples (tomato, guava, carrot, mango, acerola, papaya, and pumpkin) and presented a yield to 5.5-fold higher when compared to the reported methods indicating high robustness. Based on results, the UAE method developed has demonstrated feasibility and reliability for the study of carotenoids in buriti pulp as well as in other plant matrices with high biological relevance.

Buriti ( Mauritia flexuosa L.) 是类胡萝卜素的重要来源，但这些化合物的提取方法既费力又低效。目前的工作评估了高强度超声与化学计量学方法相结合的方法，以开发从 buriti 果肉中提取类胡萝卜素的最佳方法。多元优化通过两个步骤进行。首先，使用单纯晶格混合物设计来优化萃取溶液，发现更高的萃取产量（903 ± 21 µg g ^-1) 与丙酮:乙醇 (75/25) 混合物。之后，应用中心复合设计 (CCD) 优化样品质量 (80 mg) 和超声处理时间 (30 min)，使提取方法的收率提高了 14%。因此，最佳提取条件下的总类胡萝卜素含量 (TCC) 为 1026 ± 13 µg g ^-1 这几乎是文献中已知的 buriti 方法产量的两倍。RP-HPLC-DAD 分析表明类胡萝卜素被温和地提取，β-胡萝卜素是提取物中的主要化合物。为确认准确性，buriti 样品添加了 β-胡萝卜素标准品，开发的方法显示回收率 >84%，精密度 <6.5%。此外，优化的超声辅助提取 (UAE) 方法应用于其他样品（番茄、番石榴、胡萝卜、芒果、针叶樱桃、木瓜和南瓜），与报告的方法相比，其产量高出 5.5 倍，表明高鲁棒性。根据结果​​，阿联酋开发的方法证明了研究 buriti 果肉以及其他具有高度生物相关性的植物基质中类胡萝卜素的可行性和可靠性。