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Improvement of plant tolerance to drought stress by cotton tubby-like protein 30 through stomatal movement regulation
Journal of Advanced Research ( IF 10.7 ) Pub Date : 2022-06-20 , DOI: 10.1016/j.jare.2022.06.007
Zhanshuai Li 1 , Ji Liu 1 , Meng Kuang 2 , Chaojun Zhang 2 , Qifeng Ma 2 , Longyu Huang 2 , Huiying Wang 2 , Shuli Fan 2 , Jun Peng 1
Affiliation  

Introduction

Cotton is a vital industrial crop that is gradually shifting to planting in arid areas. However, tubby-like proteins (TULPs) involved in plant response to various stresses are rarely reported in cotton. The present study exhibited that GhTULP30 transcription in cotton was induced by drought stress.

Objective

The present study demonstrated the improvement of plant tolerance to drought stress by GhTULP30 through regulation of stomatal movement.

Methods

GhTULP30 response to drought and salt stress was preliminarily confirmed by qRT-PCR and yeast stress experiments. Ectopic expression in Arabidopsis and endogenous gene silencing in cotton were used to determine stomatal movement. Yeast two-hybrid and spilt-luciferase were used to screen the interacting proteins.

Results

Ectopic expression of GhTULP30 in yeast markedly improved yeast cell tolerance to salt and drought. Overexpression of GhTULP30 made Arabidopsis seeds more resistant to drought and salt stress during seed germination and increased the stomata closing speed of the plant under drought stress conditions. Silencing of GhTULP30 in cotton by virus-induced gene silencing (VIGS) technology slowed down the closure speed of stomata under drought stress and decreased the length and width of the stomata. The trypan blue and diaminobenzidine staining exhibited the severity of leaf cell necrosis of GhTULP30-silenced plants. Additionally, the contents of proline, malondialdehyde, and catalase of GhTULP30-silenced plants exhibited significant variations, with obvious leaf wilting. Protein interaction experiments exhibited the interaction of GhTULP30 with GhSKP1B and GhXERICO.

Conclusion

GhTULP30 participates in plant response to drought stress. The present study provides a reference and direction for further exploration of TULP functions in cotton plants.



中文翻译:

棉花管状蛋白30通过气孔运动调节提高植物对干旱胁迫的耐受性

介绍

棉花是重要的经济作物,正在逐步转向干旱地区种植。然而,棉花中很少报道涉及植物对各种胁迫的反应的桶状蛋白(TULP)。本研究表明棉花中的GhTULP30转录受干旱胁迫诱导。

客观的

本研究表明GhTULP30通过调节气孔运动提高了植物对干旱胁迫的耐受性。

方法

通过qRT-PCR和酵母胁迫实验初步证实了GhTULP30对干旱和盐胁迫的响应。拟南芥中的异位表达和棉花中的内源基因沉默被用于确定气孔运动。酵母双杂交和分裂荧光素酶用于筛选相互作用的蛋白质。

结果

GhTULP30在酵母中的异位表达显着提高了酵母细胞对盐和干旱的耐受性。GhTULP30的过表达使拟南芥种子在种子萌发过程中更能抵抗干旱和盐胁迫,并增加植物在干旱胁迫条件下的气孔关闭速度。通过病毒诱导基因沉默(VIGS)技术沉默棉花中的GhTULP30,减缓了干旱胁迫下气孔的关闭速度,降低了气孔的长度和宽度。台盼蓝和二氨基联苯胺染色显示GhTULP30沉默植物的叶细胞坏死的严重程度。此外,GhTULP30的脯氨酸、丙二醛和过氧化氢酶的含量-沉默的植物表现出显着的变异,叶片明显萎蔫。蛋白质相互作用实验展示了 GhTULP30 与 GhSKP1B 和 GhXERICO 的相互作用。

结论

GhTULP30参与植物对干旱胁迫的反应。本研究为进一步探索棉株TULP功能提供了参考和方向。

更新日期:2022-06-20
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