Here, we developed a polarization-resolved surface plasmon coupling electrochemiluminescence (ECL) strategy based on orientational Au nanobipyramids (Au NBPs) patterned structures. As the surface plasmonic materials, the Au NBPs with large localized electromagnetic field enhancement were used to construct the different patterned structures through the droplet evaporation induced assembly. The distinct morphology of the horizontal and vertical Au NBPs patterned structures provided the different plasmonic properties reflecting on resonance peak positions and polarized absorbance. As a result, the variations of the polarized ECL emission of SnS₂ QDs have been regulated by the Au NBPs patterned structures via the surface plasmon coupling progress. Because of the superior polarization-resolved ECL performances, the sensing system was used to analyze the miRNA-21 and miRNA-205 expression level in tumor tissues of triple negative breast cancer patients. The satisfactory results indicated this polarization-resolved sensing strategy possessed great potential for clinical analysis.

在这里，我们开发了一种基于定向金纳米双锥体 (Au NBPs) 图案结构的偏振分辨表面等离子体耦合电化学发光 (ECL) 策略。作为表面等离子体材料，具有大局部电磁场增强的Au NBPs被用于通过液滴蒸发诱导组装构建不同的图案化结构。水平和垂直 Au NBPs 图案结构的不同形态提供了反映共振峰位置和偏振吸光度的不同等离子体特性。结果，SnS _{2的极化 ECL 发射的变化}量子点已通过表面等离子体耦合过程由 Au NBPs 图案化结构调节。由于优异的偏振分辨 ECL 性能，该传感系统用于分析三阴性乳腺癌患者肿瘤组织中 miRNA-21 和 miRNA-205 的表达水平。令人满意的结果表明这种偏振分辨传感策略具有巨大的临床分析潜力。