Traditional disinfection methods against pathogens have numerous shortcomings, and inventive methods like cold plasma are required for virus inactivation. Here, an atmospheric pressure 13.56 MHz radio-frequency hollow cathode (RF–HC) cold plasma device has been used to build a cold plasma sterilization device, and its virucidal activity is assessed against P2 bacteriophage, a model surrogate for pathogenic viruses. The heart of this device contains a three-layered sterilization chamber which is a rectangular parallelopiped of 42 × 32 × 30 cm³. Optimization experiments were performed to make each corner of this chamber completely virus free after cold plasma treatment. This two-pronged study was conducted to establish the requirement of minimum vol. % H₂O₂ in minimum time for the complete elimination of phages inside this sterilization chamber even when plasma is not in the direct line of sight. In initial experiments, the effect of the direct plasma line of sight was seen as the top and bottom layers showing less phage killing as compared to the middle of the sterilization chamber. Complete sterilization of bacteriophage, in all the three layers inside the sterilization chamber, was achieved by plasma treatment with 6% H₂O₂ for 10 min in 80 watts of plasma operating power. It was also seen that 6% H₂O₂ mist alone is not sufficient to provide a high degree of sterilization, and normal water mist combined with cold plasma can provide a higher level of sterilization at each corner of the chamber.

传统的病原体消毒方法有很多缺点，病毒灭活需要冷等离子体等创造性方法。在这里，大气压 13.56 MHz 射频空心阴极 (RF-HC) 冷等离子体装置已被用于构建冷等离子体灭菌装置，并针对 P2 噬菌体（一种致病病毒的模型替代物）评估其杀病毒活性。该设备的核心包含一个三层灭菌室，它是一个 42 × 32 × 30 cm ³的长方体。进行优化实验以使该腔室的每个角落在冷等离子体处理后完全无病毒。进行这项两管齐下的研究是为了确定最小体积的要求。% H ₂ O ₂即使血浆不在直接视线范围内，也能在最短的时间内彻底清除灭菌室内的噬菌体。在最初的实验中，直接等离子视线的影响被视为顶层和底层与灭菌室的中间相比显示出较少的噬菌体杀伤。通过在 80 瓦等离子工作功率下用 6% H ₂ O ₂等离子处理 10 分钟，实现了灭菌室内所有三层中噬菌体的完全灭菌。还可以看出，6% H ₂ O ₂单独的水雾不足以提供高度的杀菌，而普通水雾结合冷等离子体可以在腔室的每个角落提供更高水平的杀菌。