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Measuring SARS-CoV-2 T cell immunity with a scalable qPCR-based assay
Nature Biotechnology ( IF 46.9 ) Pub Date : 2022-06-17 , DOI: 10.1038/s41587-022-01358-3


We developed two quantitative PCR-based assays to detect SARS-CoV-2-specific T cell immunity: qTACT and dqTACT. The assays quantify CXCL10 mRNA, after incubation of whole blood with viral peptides, as a proxy of an antigen-specific T cell response, and will allow population-level monitoring of cellular immunity to SARS-CoV-2.

中文翻译:

使用可扩展的基于 qPCR 的测定法测量 SARS-CoV-2 T 细胞免疫力

我们开发了两种基于 PCR 的定量检测方法来检测 SARS-CoV-2 特异性 T 细胞免疫:qTACT 和 dqTACT。在将全血与病毒肽孵育后,该检测可量化 CXCL10 mRNA,作为抗原特异性 T 细胞反应的代表,并将允许在人群水平上监测对 SARS-CoV-2 的细胞免疫力。
更新日期:2022-06-19
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