Sambucus nigra fruit and flower extracts exhibit anti-viral and anti-inflammatory properties however, the potential of S. nigra in vitro cell suspension cultures to produce biologically active compounds remains unexplored. This study describes the establishment of S. nigra cell suspension cultures and their phytochemical content and biological activity. S. nigra cell cultures contain major compounds characteristic for whole-plant S. nigra tissues albeit at uncharacteristic ratios. Elicitation with methyl jasmonate, visual selection of pigmented cells, and optimization of culture parameters resulted in cell suspensions with high contents of anthocyanins, organic acids, and phenolic compounds. Enzyme-linked immunosorbent assay (ELISA) and fluorometric assay-based assessment of biological activity of developed cultures demonstrated inhibitory capacity against cyclooxygenase enzyme COX2 activity and against the binding of SARS-CoV2 receptor-binding domain (RBD) to angiotensin-converting enzyme 2 (ACE2) in vitro suggesting that S. nigra cell suspensions exhibit anti-inflammatory and viral entry blocking potential. Differences in phytochemical content and biological activity of S. nigra cell suspensions subjected to different optimization approaches indicate potential for optimization towards desired biotechnological products with anti-inflammatory properties and inhibitory capacity against viral binding.

黑接骨木果实和花提取物具有抗病毒和抗炎特性，然而，黑莓体外细胞悬浮培养产生生物活性化合物的潜力仍未得到探索。本研究描述了S. nigra细胞悬浮培养物的建立及其植物化学成分和生物活性。S. nigra细胞培养物含有整株S. nigra特有的主要化合物组织虽然在非典型的比例。茉莉酸甲酯诱导、色素细胞的视觉选择和培养参数的优化导致细胞悬浮液中花青素、有机酸和酚类化合物的含量高。酶联免疫吸附测定 (ELISA) 和基于荧光测定的已开发培养物的生物活性评估证明了对环氧合酶 COX2 活性和 SARS-CoV2 受体结合结构域 (RBD) 与血管紧张素转换酶 2 结合的抑制能力。 ACE2)在体外表明S. nigra细胞悬液表现出抗炎和病毒进入阻断潜力。S. nigra的植物化学成分和生物活性的差异经过不同优化方法的细胞悬浮液表明，具有抗炎特性和抑制病毒结合能力的所需生物技术产品的优化潜力。