Plant oils are sources of metabolites that have enormous potential for industrial applications. Herein, the chemical profile and in vitro antimicrobial activity of the essential oil (EO) from the leaves of Guatteria citriodora Ducke (Annonaceae) have been investigated for the first time. The composition of the hydrodistilled EO was analyzed using gas chromatography-mass spectrometry (GC-MS), which permitted the identification of oxygenated monoterpenes as the most highly representative class, and included citronellal (40.99%) and citronellol (14.6%) as the main compounds. The antimicrobial activity of G. citriodora EO (GcEO) was evaluated against pathogenic bacteria and phytopathogenic fungi. The experimental design was completely randomized (CRD), and used doses for each microorganism. Gram-positive strains were the most sensitive with a minimum inhibitory concentration (MIC) of 5.0 μL mL⁻¹, while Gram-negative strains were 10.0 μL mL⁻¹. The most potent antifungal activity was against Alternaria alternata (MIC of 1.25 μL mL⁻¹). In addition, it fully inhibited A. alternata conidia germination at the minimum inhibitory concentration. The nucleic acid and soluble protein contents were significantly released from the conidia of A. alternata after treatment with GcEO. Using SEM (scanning electron microscopy), morphological alterations were observed in the conidia, which indicates that a lesion in the cytoplasmic membrane is one of its mechanisms of action. Overall, these results indicate that GcEO is an antimicrobial agent with potential applications in the agriculture, food, and pharmaceutical industries.

植物油是具有巨大工业应用潜力的代谢物的来源。在此，首次研究了来自Guatteria citriodora Ducke (Annonaceae)叶子的精油 (EO) 的化学特征和体外抗菌活性。使用气相色谱-质谱法 (GC-MS) 分析了加氢蒸馏 EO 的组成，这使得氧化单萜类成为最具代表性的类别，包括香茅醛(40.99%) 和香茅醇 (14.6%) 作为主要化合物。G的抗菌活性。柑桔属EO (GcEO) 针对病原菌和植物病原真菌进行了评估。实验设计是完全随机的（CRD），并使用每种微生物的剂量。革兰氏阳性菌株最敏感，最低抑菌浓度 (MIC) 为 5.0 μL mL ^-1，而革兰氏阴性菌株为 10.0 μL mL ^-1。最有效的抗真菌活性是对抗链格孢（MIC 为 1.25 μL mL ^-1）。此外，它完全抑制了 A。在最低抑制浓度下交替孢子萌发。A. alternata分生孢子中核酸和可溶性蛋白含量显着释放用 GceO 处理后。使用SEM（扫描电子显微镜），在分生孢子中观察到形态学改变，这表明细胞质膜中的病变是其作用机制之一。总体而言，这些结果表明 GcEO 是一种在农业、食品和制药行业具有潜在应用的抗菌剂。