To expand the applications of waste paper (WP), we attempted to valorize waste office paper (WOP) into cellobionic acid (CBA) using a two-step biocatalytic method. First, we prepared cellobiose from WOP using commercial cellulases. To increase cellobiose production, we optimized various reaction conditions; the type of cellulases, reaction temperature, the amount of WOP, and the amount of cellulases. Under the optimized reaction conditions, 23 g/L cellobiose was generated from 80 g/L of WOP. CBA was then produced from the prepared cellobiose using recombinant Pseudomonas taetrolens homologously expressing quinoprotein glucose dehydrogenase. From 23 g/L cellobiose derived from WOP, this strain produced 24.1 g/L CBA. We demonstrated for the first time that WOP could be successfully valorized into CBA using cellobiogenic cellulases and a CBA-producing strain. This study sets the groundwork to produce CBA at a low price and is expected to expand the application of CBA in industries.

为了扩大废纸（WP）的应用，我们尝试使用两步生物催化方法将废办公纸（WOP）转化为纤维酸（CBA）。首先，我们使用商业纤维素酶从 WOP 制备纤维二糖。为了提高纤维二糖的产量，我们优化了各种反应条件；纤维素酶的种类、反应温度、WOP的量、纤维素酶的量。在优化的反应条件下，80 g/L WOP 生成 23 g/L 纤维二糖。然后使用重组Pseudomonas taetrolens从制备的纤维二糖生产 CBA同源表达醌蛋白葡萄糖脱氢酶。从 WOP 衍生的 23 g/L 纤维二糖中，该菌株产生 24.1 g/L CBA。我们首次证明，使用纤维生物纤维素酶和产生 CBA 的菌株，WOP 可以成功地转化为 CBA。本研究为低价生产 CBA 奠定了基础，并有望扩大 CBA 在行业中的应用。