Atractylodes macrocephalon polysaccharides of alleviating weightlessness-induced bone loss (WIBL) are rarely reported. In this study, a neutral polysaccharide fragment named AMP1-1 was isolated and purified. Monosaccharide composition and gel permeation chromatography analysis indicated that AMP1-1 was composed of glucose and fructose with molecular weight of 1.433 kDa. Based on data of gas chromatography–mass spectrometer (GCMS), a linear backbone consisted of α-d-Glcp-(1→ and →1)-β-d-Fruf-2→ was discovered. Combining results from nuclear magnetic resonance (NMR), the inulin-type fructan AMP1-1 was identified as α-d-Glcp-1→(2-β-d-Fruf-1)₇. Anti-WIBL activity of AMP1-1 was evaluated though analyzation of mechanical properties, BALP and TRAP 5b activities on femur. In vitro mRNA expression indicated that anti-WIBL activity of AMP1-1 was achieved by promote bone formation and inhibit bone resorption in primary osteoblasts and RAW264.7 cell lines under simulated weightlessness. In conclusion, the inulin-type fructan AMP1-1 with α-d-Glcp-1→(2-β-d-Fruf-1)₇ had anti-WIBL activity via remodeling bone homeostasis.

很少报道白术多糖减轻失重引起的骨质流失 (WIBL) 。在本研究中，分离纯化了一种名为 AMP1-1 的中性多糖片段。单糖组成和凝胶渗透色谱分析表明AMP1-1由葡萄糖和果糖组成，分子量为1.433 kDa。基于气相色谱-质谱仪 (GCMS) 的数据，发现了由 α- d -Glc p -(1→ 和 →1)-β- d -Fru f -2→ 组成的线性骨架。结合核磁共振 (NMR) 的结果，菊粉型果聚糖 AMP1-1 被鉴定为 α- d -Glc p -1→(2-β- d -Fruf -1) ₇ . 通过分析股骨上的机械性能、BALP 和 TRAP 5b 活性来评估 AMP1-1 的抗 WIBL 活性。体外 mRNA 表达表明，AMP1-1 的抗 WIBL 活性是通过在模拟失重条件下促进原代成骨细胞和 RAW264.7 细胞系的骨形成和抑制骨吸收来实现的。总之，具有α-d-Glcp-1→(2-β-d-Fruf-1) 7 的菊粉型果聚糖AMP1-1通过重塑骨稳态_具有抗WIBL活性。