Glutathione (GSH) plays important roles in various physiological processes, thus highly sensitive assay of GSH and timely warning of its variation at trace level in complex biological matrixes is of great significance. However, this is challenging due to the coexisting reductive biomolecules and dynamic change of GSH levels in responding to various stimuli which remain largely unexploited. Herein, we report a dual mode protocol for the assay of GSH based on nanoconjugate g-C₃N₄:Tb/MnO₂ between MnO₂ nanosheets and terbium-doped g-C₃N₄ (g-C₃N₄:Tb) nanosheets. MnO₂ moiety effectively quenches the emission at 546 nm from Tb³⁺ in the nanoconjugate, which is restored under the reduction of MnO₂ by GSH to ensure fluorescence turn-on assay of GSH. Meanwhile, the generated Mn²⁺ facilitates inductively coupled plasma mass spectrometry (ICP-MS) detection to endow indirect highly sensitive assay of GSH. Fluorescence mode derived a limit of detection (LOD) of 0.17 μmol L⁻¹ within a linear range of 0.5–160 μmol L⁻¹, while ICP-MS resulted in a superior LOD of 0.016 μmol L⁻¹ within 0.05–160 μmol L⁻¹. Both detection modes provide excellent selectivity to GSH. The dual mode platform was validated by GSH assay in cell lysates. It was further demonstrated by monitoring the variation of dynamic change of GSH level under CuSO₄ or cisplatin induced GSH consumption.

谷胱甘肽（GSH）在多种生理过程中发挥着重要作用，因此对GSH进行高灵敏度测定并及时预警其在复杂生物基质中的痕量变化具有重要意义。然而，这是具有挑战性的，因为还原性生物分子并存，谷胱甘肽水平在响应各种刺激时动态变化，而这些刺激在很大程度上仍未被利用。_{在此，我们报告了一种基于 MnO 2}纳米片和掺铽的 gC ₃ N ₄ (gC ₃ N ₄ :Tb) 纳米片之间的纳米共轭 gC ₃ N ₄ :Tb/MnO _{2测定 GSH 的双模式方案。二氧化}锰^{基团有效地猝灭纳米缀合物中 Tb 3+}在546 nm 处的发射，在GSH还原 MnO ₂下恢复发射，以确保 GSH 的荧光开启测定。同时，生成的Mn ²⁺有利于电感耦合等离子体质谱（ICP-MS）检测，赋予GSH间接高灵敏度测定。^{荧光模式在 0.5-160 μmol L -1}的线性范围内得出0.17 μmol L ^-1的检测限 (LOD) ，而 ICP-MS在 0.05-160 μmol L -1 范围内产生了 0.016 μmol L ^{-1的出色 LOD -1}. 两种检测模式均可对 GSH 提供出色的选择性。通过细胞裂解物中的 GSH 测定验证了双模式平台。_{通过监测CuSO 4}或顺铂诱导的GSH消耗下GSH水平的动态变化进一步证明了这一点。