The inactivation of antibiotic resistant bacteria (ARB) and genes (ARGs) in an advanced plant combining ozonation and granular activated carbon (GAC) filtration applied for effluent after conventional activated sludge treatment at a full-scale urban wastewater treatment plant was investigated for over 13 consecutive months. The nitrite compensated specific ozone dose ranged between 0.4 and 0.7 g O₃/g DOC with short-time sampling campaigns (0.2–0.9 g O₃/g DOC). Samples were analysed with culture-dependent methods for bacterial targets and with qPCR for genes. The log removal values were correlated with a decrease of the matrix UV absorption at 254 nm (ΔUV₂₅₄) and indicated a range of ΔUV₂₅₄ that corresponds to a sufficient membrane damage to affect DNA. For trimethoprim/sulfamethoxazole resistant E. coli, sul1, ermB and tetW, this phase was observed at ΔUV₂₅₄ of ~30 % (~0.5 g O₃/g DOC). For ampicillin resistant E. coli and bla_TEM-1, it was observed around 35–40 % (~0.7 g O₃/g DOC), which can be linked to mechanisms related to oxidative damages in bacteria resistant to bactericidal antibiotics. GAC treatment resulted in a further abatement for trimethoprim/sulfamethoxazole E. coli, sul1 and tetW, and in increase in absolute and relative abundance of ermB and bla_TEM-1.

在一个综合了臭氧和颗粒活性炭 (GAC) 过滤的先进工厂中，抗生素抗性细菌 (ARB) 和基因 (ARGs) 的灭活被调查了超过 13 年连续几个月。亚硝酸盐补偿的特定臭氧剂量范围在 0.4 和 0.7 g O ₃ /g DOC 之间，采用短时间采样活动 (0.2–0.9 g O ₃ /g DOC)。使用细菌靶标的培养依赖性方法和基因的 qPCR 分析样品。对数去除值与基质在 254 nm 处的 UV 吸收 (ΔUV ₂₅₄ ) 的降低相关，并表明 ΔUV _254的范围这对应于足以影响 DNA 的膜损伤。对于甲氧苄啶/磺胺甲恶唑耐药的大肠杆菌、sul 1、erm B 和tet W，在 ΔUV ₂₅₄为 ~30 % (~0.5 g O ₃ /g DOC) 时观察到该阶段。对于氨苄青霉素耐药的大肠杆菌和bla _TEM-1，观察到大约 35-40%（~0.7 g O ₃ /g DOC），这可能与对杀菌抗生素耐药的细菌的氧化损伤机制有关。GAC 处理进一步减少了甲氧苄啶/磺胺甲恶唑大肠杆菌、sul 1 和tetW，并且erm B 和bla _TEM-1的绝对和相对丰度增加。