Arginine and its metabolites play important roles in pain and analgesia. This study aimed to develop a comprehensive quantification method for amino acids and metabolites related to arginine metabolism in rat plasma by hydrophilic interaction chromatography–tandem mass spectrometry (HILIC–MS/MS). Rat plasma was diluted to reduce the matrix effect and deproteinized with acetonitrile. The analytes were separated on a Syncronis HILIC column with a gradient elution. MS analysis was performed in positive ion mode with an electrospray ionization source using multiple reaction monitoring technology. All calibration curves for the 10 analytes showed good linear regression (R2 > 0.99). The limits of detection (LODs) were in the range of 0.9–13.4 μg/L. The established method was validated for intra-day and inter-day precisions (relative standard deviation [RSDs] < 6.21%) and accuracy (average recovery ranged from 87.34% to 100.35% with the RSD values less than 11.41%). This method was successfully applied to characterize dynamic alterations in the plasma of rats with neuropathic pain and thus provide service to explore the mechanism of action between metabolite changes and clinical disease.

中文翻译：

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通过亲水相互作用色谱-串联质谱法同时测定大鼠血浆中与精氨酸代谢相关的代谢物

精氨酸及其代谢物在疼痛和镇痛中起重要作用。本研究旨在通过亲水相互作用色谱-串联质谱法 (HILIC-MS/MS) 开发大鼠血浆中与精氨酸代谢相关的氨基酸和代谢物的综合定量方法。大鼠血浆被稀释以减少基质效应并用乙腈脱蛋白。分析物在 Syncronis HILIC 色谱柱上通过梯度洗脱进行分离。使用多反应监测技术，在电喷雾电离源的正离子模式下进行 MS 分析。10 种分析物的所有校准曲线都显示出良好的线性回归 (R2 > 0.99)。检测限 (LOD) 在 0.9–13.4 μg/L 的范围内。已建立的方法针对日内和日间精密度（相对标准偏差 [RSD] < 6.21%）和准确度（平均回收率范围为 87.34% 至 100.35%，RSD 值小于 11.41%）进行了验证。该方法成功应用于表征神经性疼痛大鼠血浆的动态变化，从而为探索代谢物变化与临床疾病之间的作用机制提供服务。